Abstract
In the present investigation we studied the extent of variation among barley genotypes (Hordeum vulgare L. cv. Alexis, Canut, Digger, Etna, Peel) in their ability: i) to induce activity of soluble extracellular phosphatase in rhizosphere soil. ii) to withdraw bicarbonate extractable organic phosphorus (NaHCO3-P0). All the genotypes induced 3–4 times higher phosphatase activities in rhizosphere soil as compared to bulk soil. Among the genotypes, there were significant (p>0.01) differences in soluble extracellular and non-soluble phosphatase activities and depletion of NaHCO3-P0 in soil near their root mats. Etna induced highest phosphatase activities and depleted most NaHCO3-P0 from the rhizosphere soil. A high correlation (r=0.79) was found between the activity of soluble extracellular phosphatase and the quantity of NaHCO3-P0 withdrawn from the rhizosphere soil by the barley genotypes.
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References
Asmar F, Eiland F and Nielsen N E 1992 Interrelationship between extracellular enzyme activity, ATP content, total counts of bacteria and CO2 evolution. Biol. Fertil. Soils 14, 288–292.
Asmar F 1992 The role of extracellular enzymes and microbial biomass in the process of nitrogen mineralization in soil. Ph.D. thesis, The Royal Veterinary and Agricultural University, Copenhagen.
Barber D A and Lynch J M 1976 Microbial growth in the rhizosphere. Soil Biol. Biochem. 9, 305–308.
Brown M G and Tabatabai M A 1978 Phosphodiesterase activity of soil. Soil Sci. Soc. Am. J. 42, 284–290.
Burns R G 1982 Enzyme activity in soil: Location and a possible role in microbial ecology. Soil Biol. Biochem. 12, 423–427.
Doumas P, Berjaud C, Calleja M, Coupe M, Espfau C and d'Ausac J 1986 Phosphatases extracellulaires et nutrition phosphate chez les champignons et les plantes hotes. Physiol. Veg. 24, 173–184.
Ebina J, Tsutsui T and Shirai T 1983 Simultaneous determination of total nitrogen and total phosphorus in water using peroxodisulphate oxidation. Water Res. 17, 721–726.
Eivazi F and Tabatabai M A 1977 Phosphatase in soils. Soil Biol. Biochem. 9, 167–172.
Gahoonia T S and Nielsen N E 1991 A method to study rhizosphere processes in thin soil layers of different proximity to roots. Plant and Soil 135, 143–146.
Gahoonia T S and Nielsen N E 1992a Control of pH at the soil-root interface. Plant and Soil 140, 49–54.
Gahoonia T S and Nielsen N E 1992b The effect of root-induced pH changes on the depletion of inorganic and organic phosphorus in the rhizosphere. Plant and Soil 143, 185–191.
Gahoonia T S and Nielsen N E 1995 Variation among wheat and barley genotypes in their ability to withdraw phosphorus from the rhizosphere soil. Plant and Soil (Submitted).
Haussling M and Marschner H 1989 Organic and inorganic soil phosphates and acid phosphatase activity in the rhizosphere of 80-year-old Norway spruce (Picea abies L. Karst.) trees. Biol. Fertil. Soils 8, 128–133.
Hawkes G E, Powlson D S, Randall E W and Tate K R 1984 A 31P nuclear magnetic resonance study of the phosphorus species in alkali extracts of soils from long-term field experiments. J. Soil Sci. 35, 35–45.
Helal H M and Sauerbeck D R 1988 Relationship between P supply and phosphatase activity in roots of soil-grown maize (Zea mays L.). VDLUFA-Schriftenr. 23, 195–202.
Juma N G and Tabatabai M A 1988 Comparison of kinetic and thermodynamic parameters of phosphomonoesterases of soils and of corn and soybean roots. Soil Biol. Biochem. 20, 533–539.
Mousain D and Salsac L 1986 Utilisation du phytate et activities phosphatases acides chez Pisolithus tinctorius, Basidiomycete Mycorhizien. Physiol. Veg. 24, 193–200.
Murphy J and Riley J P 1962 A modified single solution method for the determination of phosphates in natural water. Anal. Chim. Acta 27, 31–36.
Rogers T H, Pearson R W and Pierre W H 1940 Absorption of organic phosphorus by corn and tomato plants and the mineralizing action of exo-enzymes systems of growing roots. Soil Sci. Soc. Proc. 5, 285–291.
Sharpley A N 1985 Phosphorus cycling in unfertilized and fertilized agricultural soils. Soil Sci. Soc. Am. J. 49, 905–911.
Silberbush M, Shomer-Ilan A and Waisel Y 1981 Root surface phosphatase activity in ecotypes of Aegilops peregrina. Physiol. Plant. 53, 501–504.
Tabatabai M A and Bremner J M 1969 Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 301–307.
Tarafdar J C and Jungk A 1987 Phosphatase activity in rhizosphere and its relation to the depletion of soil organic phosphorus. Biol. Fertil. Soils 3, 199–204.
Tarafdar J C and Claassen N 1988 Organic phosphorus compounds as a phosphorus source for higher plant through the activity of phosphatase produced by plant roots and microorganisms. Biol. Fertil. Soils 5, 308–312.
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Asmar, F., Singh, T., Gahoonia et al. Barley genotypes differ in activity of soluble extracellular phosphatase and depletion of organic phosphorus in the rhizosphere soil. Plant Soil 172, 117–122 (1995). https://doi.org/10.1007/BF00020865
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DOI: https://doi.org/10.1007/BF00020865