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Protective Role of Black Tea Flavonoids Against Ethanol-Induced Gastropathy via Matrix Metalloproteinase Pathway

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Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Tea polyphenols are known to prevent various ailments like cancer, atherosclerosis, hypertension and diabetes. Our study aimed at to decipher the gastroprotective effect of aqueous black tea extract (BTE) against ethanol-induced gastric damage and the role of BTE in modulating MMP-9 activity and expression, both in vivo and in vitro. The protective role of BTE was assessed in Sprague–Dawley rats after inducing damage with 70% ethanol. Human gastric adenocarcinoma cells (AGS) were treated with ethanol in ex vivo experiment. MMP-9 activity and expression were investigated through gelatin zymography and western blotting. Reactive oxygen species (ROS) generation was also studied by fluorescence spectroscopy and confocal microscopy, with or without treatment of BTE both in vivo and in vitro experiments. In addition, the effect of citric acid treated BTE (cBTE), which mimics lemon tea, was examined on ethanol-induced gastropathy. BTE exhibited antiulcer activity through reduction of glutathione depletion, lipid peroxidation, protein oxidation, ROS production and inflammatory cell infiltration in rat gastric tissues. In addition, BTE significantly inhibited synthesis and secretion of proMMP-9 both in vivo and in vitro. The mitochondrial enzymes succinate dehydrogenase and NADH oxidase in rat gastric tissues were downregulated by BTE while protecting gastric ulcer. Citric acid addition to BTE was observer to enrich the lead compound, catechin. Interestingly, cBTE showed higher anti-ulcer activity than the untreated one. BTE shows protective role against ethanol-induced gastric ulcer in rats through scavenging ROS and downregulating proMMP-9 activity. While cBTE shows better protection due to enrichment of catechin and removal of tannins in tea extract leading to enhanced inhibitory role on proMMP-9 activity and ROS production.

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Abbreviations

BTE:

Black tea extract

cBTE:

Citric acid treated black tea extract

ROS:

Reactive oxygen species

MMP:

Matrix metalloproteinase

ECM:

Extracellular matrix

SDH:

Succinate dehydrogenase

TIMP:

Tissue inhibitor of metalloproteinases

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Acknowledgements

This work was financed by the CSIR Network Projects HUM (BSC 0119) and INDEPTH (BSC 0111). Mr. Diptadeep Sarkar and Mr. T. Muruganandan are thankfully acknowledged for their assistance in confocal microscopy and scanning electron microscopy (SEM).

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Author notes

  1. Susri Raychaudhuri and Satyabrata Ghosh have contributed equally to this work.

Authors and Affiliations

  1. Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, Kolkata, 700 032, India

    Susri Raychaudhuri, Anirban Roy & Snehasikta Swarnakar

  2. Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata, 700 032, India

    Satyabrata Ghosh

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  1. Susri Raychaudhuri
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  2. Satyabrata Ghosh
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Correspondence to Snehasikta Swarnakar.

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Raychaudhuri, S., Ghosh, S., Roy, A. et al. Protective Role of Black Tea Flavonoids Against Ethanol-Induced Gastropathy via Matrix Metalloproteinase Pathway. Ind J Clin Biochem 34, 379–394 (2019). https://doi.org/10.1007/s12291-018-0762-x

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