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Transcriptome analysis to understand the effects of the toxoflavin and tropolone produced by phytopathogenic Burkholderia on Escherichia coli

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

The phytopathogenic Burkholderia species B. glumae and B. plantarii are the causal agents of bacterial wilt, grain rot, and seedling blight, which threaten the rice industry globally. Toxoflavin and tropolone are produced by these phytopathogens and are considered the most hostile biohazards with a broad spectrum of target organisms. However, despite their nonspecific toxicity, the effects of toxoflavin and tropolone on bacteria remain unknown. RNA-seq based transcriptome analysis was employed to determine the genome-wide expression patterns under phytotoxin treatment. Expression of 2327 and 830 genes was differentially changed by toxoflavin and tropolone, respectively. Enriched biological pathways reflected the down-regulation of oxidative phosphorylation and ribosome function, beginning with the inhibition of membrane biosynthesis and nitrogen metabolism under oxidative stress or iron starvation. Conversely, several systems such as bacterial chemotaxis, flagellar assembly, biofilm formation, and sulfur/taurine transporters were highly expressed as countermeasures against the phytotoxins. In addition, our findings revealed that three hub genes commonly induced by both phytotoxins function as the siderophore enterobactin, an iron-chelator. Our study provides new insights into the effects of phytotoxins on bacteria for better understanding of the interactions between phytopathogens and other microorganisms. These data will also be applied as a valuable source in subsequent applications against phytotoxins, the major virulence factor.

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Acknowledgements

This study was financially supported by the ‘2018 Post-Doc. Development Program’ of Pusan National University. This research was supported by grants from the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (No. 916009021SB010).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology, Pusan National University, Busan, 46241, Republic of Korea

    Jungwook Park, Hyun-Hee Lee, Hyejung Jung & Young-Su Seo

  2. Bioinformation Technology Division, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju, 37242, Republic of Korea

    Jungwook Park

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  1. Jungwook Park
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Correspondence to Young-Su Seo.

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Park, J., Lee, HH., Jung, H. et al. Transcriptome analysis to understand the effects of the toxoflavin and tropolone produced by phytopathogenic Burkholderia on Escherichia coli. J Microbiol. 57, 781–794 (2019). https://doi.org/10.1007/s12275-019-9330-1

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