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Exosome-Derived Dystrophin from Allograft Myogenic Progenitors Improves Cardiac Function in Duchenne Muscular Dystrophic Mice

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Abstract

Progressive cardiomyocyte loss in Duchenne muscular dystrophy (DMD) leads to cardiac fibrosis, cardiomyopathy, and eventually heart failure. In the present study, we observed that myogenic progenitor cells (MPC) carry mRNA for the dystrophin gene. We tested whether cardiac function can be improved in DMD by allograft transplantation of MPC-derived exosomes (MPC-Exo) into the heart to restore dystrophin protein expression. Exo from C2C12 cells (an MPC cell line) or vehicle were delivered locally into the hearts of MDX mice. After 2 days of treatment, we observed that MPC-Exo restored dystrophin expression in the hearts of MDX mice, which correlated with improved myocardial function in dystrophin-deficient MDX mouse hearts. In conclusion, this study demonstrated that allogeneic WT-MPC-Exo transplantation transiently restored dystrophin gene expression and improved cardiac function in MDX mice, suggesting that allogenic exosomal delivery may serve as an alternative treatment for cardiomyopathy of DMD.

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Funding

I. Kim, N.L. Weintraub, and Y. Tang were partially supported by the American Heart Association: GRNT31430008, NIH-AR070029, NIH-HL086555, NIH-HL134354, and NIH -HL12425.

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Author notes

  1. Xuan Su and Yue Jin contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 160, Pujian Road, Shanghai, 200127, China

    Xuan Su, Yue Jin, Yu Wang & Meng Jiang

  2. Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA

    Yan Shen, Jingwen Cai, Yutao Liu, Il-man Kim, Neal L. Weintraub & Yaoliang Tang

  3. Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, China

    Chengwei Ju

  4. Department of Cardiology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, China

    Hong Yu

Authors
  1. Xuan Su
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  2. Yue Jin
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  3. Yan Shen
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  4. Chengwei Ju
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  5. Jingwen Cai
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  6. Yutao Liu
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  7. Il-man Kim
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  8. Yu Wang
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  9. Hong Yu
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  10. Neal L. Weintraub
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  11. Meng Jiang
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  12. Yaoliang Tang
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Corresponding authors

Correspondence to Meng Jiang or Yaoliang Tang.

Ethics declarations

This article does not contain any studies with human participants performed by any of the authors.

Animals were handled according to approved protocols and animal welfare regulations of the Institutional Animal Care and Use Committee of the Medical College of Georgia/Augusta University.

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Associate Editor Enrique Lara-Pezzi oversaw the review of this article

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Su, X., Jin, Y., Shen, Y. et al. Exosome-Derived Dystrophin from Allograft Myogenic Progenitors Improves Cardiac Function in Duchenne Muscular Dystrophic Mice. J. of Cardiovasc. Trans. Res. 11, 412–419 (2018). https://doi.org/10.1007/s12265-018-9826-9

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  • DOI: https://doi.org/10.1007/s12265-018-9826-9

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