Abstract
Objective
To examine the effect of neuropeptide Y (NPY) on TGF-β1 production in RAW264.7 macrophages.
Methods
Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-β1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85.
Results
NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β1 production induced by NPY could be abolished by wortmannin pretreatment.
Conclusion
NPY may elicit TGF-β1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.
摘要
目的
探讨神经肽Y对巨噬细胞系RAW264.7分泌转化生长因子TGF-β1的影响。
方法
酶联免疫吸附实验检测细胞培养上清液中的TGF-β1 水平; 细胞计数试剂盒CCK-8 检测细胞活力; Western blot 检测磷脂酰肌醇3 激酶PI3K p85的磷酸化水平。
结果
神经肽Y主要通过激活其Y1受体信号通路增加RAW264.7细胞中TGF-β1的产生, 并可在10 min内快速激活PI3K通路。 PI3K通路阻滞剂可消除NPY 对TGF-β1产生的促进作用。
结论
神经肽Y能够通过Y1 受体促进RAW264.7 细胞的TGF-β1 的表达, 此作用可能由PI3K介导。
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Zhou, JR., Xu, Z. & Jiang, CL. Neuropeptide Y promotes TGF-β1 production in RAW264.7 cells by activating PI3K pathway via Y1 receptor. Neurosci. Bull. 24, 155–159 (2008). https://doi.org/10.1007/s12264-008-0130-6
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DOI: https://doi.org/10.1007/s12264-008-0130-6