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A Novel Cell Disruption Approach: Effectiveness of Laser-induced Cell Lysis of Pichia pastoris in the Continuous System

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Abstract

In biotechnological processes, often cell disruption has been an inevitable step as current host cells express most of the desired products intracellularly. Thus, an appropriate cell disruption technique must be selected considering different factors including the target product, process scale, and cell wall structure. In the current study, as a novel method, the efficacy of cell disruption via laser was tested qualitatively and quantitatively in batch and continuous systems, respectively. Laser-induced cell lysis can be a clean, rapid and convenient alternative to the other conventional disruption techniques. Our investigations in the continuous system with a flow rate of 800 μL/sec proved efficient (~ 90%) Pichia pastoris cell disruption at the wavenumber 1,064 nm with the energy input of 284 mW after four complete rounds of circulation. The main mechanism of cell disruption is assumed to be thermolysis via instant heat increase in the laser-treated spot. The results of the current study showed that continuous laser system could be applied in laboratory and industry scale for cell disruption.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, University of Guilan, Rasht, Iran

    Maryam Gazor & Seyed Siamak Ashraf Talesh

  2. Production and Research Complex, Department of Recombinant Products, Pasteur Institute of Iran, Tehran, Iran

    Alireza kavianpour, Maryam Khatami, Amin Javidanbardan & Seyed Nezamedin Hosseini

Authors
  1. Maryam Gazor
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  2. Seyed Siamak Ashraf Talesh
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  3. Alireza kavianpour
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  4. Maryam Khatami
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  5. Amin Javidanbardan
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  6. Seyed Nezamedin Hosseini
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Correspondence to Seyed Nezamedin Hosseini.

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Gazor, M., Talesh, S.S.A., kavianpour, A. et al. A Novel Cell Disruption Approach: Effectiveness of Laser-induced Cell Lysis of Pichia pastoris in the Continuous System. Biotechnol Bioproc E 23, 49–54 (2018). https://doi.org/10.1007/s12257-017-0261-6

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  • DOI: https://doi.org/10.1007/s12257-017-0261-6

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