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Priming Potato with Thiamin to Control Potato Virus Y

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Abstract

Potato virus Y (PVY) is a major potato pathogen affecting potato yields worldwide. Thiamin, a water-soluble B vitamin (vitamin B1) has been shown to boost the plant’s immunity, thereby increasing resistance against pathogens. In this study, we tested different concentrations of thiamin (1 mM, 10 mM, 50 mM, 100 mM) and multiple thiamin applications (once, biweekly and monthly) on potato resistance to PVY in Ranger Russet potatoes. Plants were mechanically inoculated with PVYN:O. This PVY strain is known for causing well-defined foliar symptoms. We collected leaflets weekly through April and May 2015 and tested them with an enzyme-linked immunosorbent assay specific to PVY as well as by real time quantitative RT-PCR. These assays allowed us to determine the presence and level of PVY in different parts of the plants. We found that the highest thiamin concentration treatment (100 mM) produced the lowest virus level in potatoes across all dates and leaflet samples. Also, it was found that multiple applications of thiamin had a positive effect on reducing virus level, especially when thiamin was applied every four weeks.

Resumen

El Virus Y de la Papa (PVY) es un patógeno importante que afecta los rendimientos de la papa mundialmente. Se ha demostrado que la tiamina, una vitamina B soluble en agua (vitamina B1), estimula la inmunidad de la planta, aumentando la resistencia contra patógenos. En este estudio, probamos diferentes concentraciones de tiamina (1 mM, 10 mM, 50 mM, 100 mM) y aplicaciones múltiples (una vez, quincenalmente y mensualmente) sobre la resistencia de la papa al PVY en la variedad Ranger Russet. Las plantas se inocularon mecánicamente con PVYN:O. Esta variante es conocida por causar síntomas foliares bien definidos. Colectamos folíolos semanalmente durante abril y mayo, 2015, y los probamos con un ensayo serológico de enzimas conjugadas específico para PVY, así como con PCR-RT de tiempo real cuantitativo. Estos ensayos nos permitieron determinar la presencia y nivel de PVY en diferentes partes de la planta. Encontramos que el tratamiento con la más alta concentración de tiamina (100 mM) produjo el más bajo nivel de virus en papa a lo largo de todas las fechas y muestras de folíolos. También se encontró que múltiples aplicaciones de tiamina tuvieron un efecto positivo en la reducción del nivel del virus, especialmente cuando la tiamina se aplicó cada cuatro semanas.

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Acknowledgements

The authors would like to thank the Oregon State University Agricultural Research Foundation for funding part of this trial. We would also like to thank Matthew Warman for his help during PVY inoculation and ELISA testings, and Mark Barnett for his technical help with the real-time quantitative RT-PCR experiments.

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Author notes

  1. Amber C. Vinchesi

    Present address: University of California Cooperative Extension, 100 Sunrise Blvd. Suite E, Colusa, CA, 95932, USA

Authors and Affiliations

  1. Department of Crop and Soil Science, Hermiston Agricultural Research and Extension Center, Oregon State University, 2121 South First Street, Hermiston, OR, 97838, USA

    Amber C. Vinchesi & Silvia I. Rondon

  2. Department of Botany and Plant Pathology, Hermiston Agricultural Research and Extension Center, Oregon State University, 2121 South First Street, Hermiston, OR, 97838, USA

    Aymeric Goyer

Authors
  1. Amber C. Vinchesi
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  2. Silvia I. Rondon
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  3. Aymeric Goyer
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Correspondence to Aymeric Goyer.

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Vinchesi, A.C., Rondon, S.I. & Goyer, A. Priming Potato with Thiamin to Control Potato Virus Y. Am. J. Potato Res. 94, 120–128 (2017). https://doi.org/10.1007/s12230-016-9552-2

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