Abstract
Introduction
Controlling gene expression is a fundamental goal of basic and synthetic biology because it allows insight into cellular function and control of cellular activity. We explored the possibility of generating an optogenetic repressor of gene expression in the model organism Saccharomyces cerevisiae by using light to control the nuclear localization of nuclease-dead Cas9, dCas9.
Methods
The dCas9 protein acts as a repressor for a gene of interest when localized to the nucleus in the presence of an appropriate guide RNA (sgRNA). We engineered dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool to control nuclear localization (LINuS) as parts in an existing yeast optogenetic toolkit. This allowed expression cassettes containing novel dCas9 repressor configurations and guide RNAs to be rapidly constructed and integrated into yeast.
Results
Our library of repressors displays a range of basal repression without the need for inducers or promoter modification. Populations of cells containing these repressors can be combined to generate a heterogeneous population of yeast with a 100-fold expression range. We find that repression can be dialed modestly in a light dose- and intensity-dependent manner. We used this library to repress expression of the lanosterol 14-alpha-demethylase Erg11, generating yeast with a range of sensitivity to the important antifungal drug fluconazole.
Conclusions
This toolkit will be useful for spatiotemporal perturbation of gene expression in Saccharomyces cerevisiae. Additionally, we believe that the simplicity of our scheme will allow these repressors to be easily modified to control gene expression in medically relevant fungi, such as pathogenic yeasts.
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Acknowledgments
The authors would like to acknowledge discussion and helpful comments from the members of the McClean lab throughout the project. We acknowledge Taylor Scott for help analyzing the growth curve data, Kieran Sweeney for supplying MATLAB code to analyze nuclear localization, and Jidapas (My) An-Adirekkun for assistance with figures. This work was supported by the American Cancer Society [IRG-15-213-51] to M.N.M and by the BMBF 031L0079 grant to B.D.V. Flow cytometry was enabled by the University of Wisconsin Carbone Cancer Center Support Grant P30 CA014520. Megan Nicole McClean, Ph.D., holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.
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Authors Stephanie H. Geller, Enoch B. Antwi, Barbara Di Ventura and Megan N. McClean declare that they have no conflicts of interest.
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Megan N. McClean is an Assistant Professor in the Department of Biomedical Engineering at the University of Wisconsin-Madison. She received her B.A. from the University of California-Berkeley and her Ph.D. from Harvard University, both in Applied Mathematics. During her thesis work with Dr. Sharad Ramanathan, she used computational modeling in combination with single-cell microscopy to understand the mechanisms of crosstalk prevention and signaling specificity in Saccharomyces cerevisiae MAP kinase pathways. Prior to joining UW-Madison, Dr. McClean was a Lewis-Sigler Fellow at Princeton University where she utilized optogenetics, control theory, and synthetic biology to develop tools for controlling biological circuits. At UW-Madison, Dr. McClean’s research group employs systems and synthetic biology approaches to understand biological signal processing in fungi, including human fungal pathogens, with implications for improving treatment strategies. Dr. McClean holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund and a Maximizing Investigators’ Research Award from the National Institute of General Medical Sciences.
This article is part of the CMBE 2019 Young Innovators special issue.
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Geller, S.H., Antwi, E.B., Di Ventura, B. et al. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. Cel. Mol. Bioeng. 12, 511–528 (2019). https://doi.org/10.1007/s12195-019-00598-9
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DOI: https://doi.org/10.1007/s12195-019-00598-9