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Identification and expression analysis of a heat-shock protein 70 gene in Polycelis sp.

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Cell Stress and Chaperones Aims and scope

Abstract

Heat-shock protein 70 (HSP70) is ubiquitously found in a variety of organisms and plays an important role in cytoprotection, environmental monitoring, and disease resistance. In this study, the full-length complementary DNA (cDNA) of hsp70 from planarian Polycelis sp. was first cloned using rapid amplification of cDNA ends (RACE). The expression levels of Pyhsp70 were analyzed in the presence of various stressors by real-time PCR, and its temporal-spatial expression patterns were also examined in both intact and regenerative animals by whole-mount in situ hybridization. The results show that (1) the deduced amino acid sequence of Pyhsp70 includes three typical HSP70 family signature motifs and is highly conserved during evolution; (2) Pyhsp70 expression is induced by prolonged starvation, tissue damage, and ionic liquid but inhibited by high or low temperatures; and (3) Pyhsp70 mRNA is mainly expressed in the head peripheral region and in the regenerating blastema during regeneration. These results suggest that the highly expressed Pyhsp70 gene may contribute to enhance cytoprotection and tolerance against stress-induced molecular damage, and the migration of neoblasts to the wound, which might also be involved in the proliferation and differentiation of neoblasts. Our work provides basic data for the study of stress responses and regenerative mechanism in freshwater planarians.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Nos. 31471965, 31170357, 30870368, 30670247, 30170119), the Ph.D. Programs Foundation of the Ministry of Education of China (No. 200804760003), the Outstanding Young Scientists Foundation of Henan Province (No. 0312001100), the Innovation Foundation of Henan Province (No. 2005126), the Basic and Advanced Technique Research Program of Technology Department of Henan Province (Nos. 122300410142, 142300410160), and the Doctoral Scientific Research Start-up Foundation of Henan Normal University (No. 521).

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    1. College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, China

      Fangfang Cheng, Zimei Dong, Yanping Dong, Yingxu Sima, Jing Chen, Xiaoyan Li, Guangwen Chen & Dezeng Liu

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    1. Fangfang Cheng
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    2. Zimei Dong
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    Correspondence to Guangwen Chen.

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    GenBank accession no. KP027180

    Fangfang Cheng and Zimei Dong contributed equally to this work.

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    Cheng, F., Dong, Z., Dong, Y. et al. Identification and expression analysis of a heat-shock protein 70 gene in Polycelis sp.. Cell Stress and Chaperones 20, 907–915 (2015). https://doi.org/10.1007/s12192-015-0608-x

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