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Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

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Cell Stress and Chaperones Aims and scope

An Erratum to this article was published on 06 November 2013

Abstract

Upregulation of small heat-shock proteins (sHsps) in response to cellular stress is one mechanism to increase cell viability. We previously described that cultured rat hippocampal neurons express five of the 11 family members but only upregulate two of them (HspB1 and HspB5) at the protein level after heat stress. Since neurons have to cope with many other pathological conditions, we investigated in this study the expression of all five expressed sHsps on mRNA and protein level after sublethal sodium arsenite and oxidative and hyperosmotic stress. Under all three conditions, HspB1, HspB5, HspB6, and HspB8 but not HspB11 were consistently upregulated but showed differences in the time course of upregulation. The increase of sHsps always occurred earlier on mRNA level compared with protein levels. We conclude from our data that these four upregulated sHsps (HspB1, HspB5, HspB6, HspB8) act together in different proportions in the protection of neurons from various stress conditions.

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Acknowledgments

We would like to thank Bianca Mekle, Diana Reinhardt, and Silke Zschemisch for their expert technical assistance.

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  1. Institute of Anatomy and Cell Biology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany

    Britta Bartelt-Kirbach & Nikola Golenhofen

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  1. Britta Bartelt-Kirbach
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  2. Nikola Golenhofen
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Correspondence to Nikola Golenhofen.

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Bartelt-Kirbach, B., Golenhofen, N. Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons. Cell Stress and Chaperones 19, 145–153 (2014). https://doi.org/10.1007/s12192-013-0452-9

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