Abstract
The presence of an α-crystallin domain documents the evolutionary relatedness of the ubiquitous family of small heat shock proteins. Sequence and three-dimensional structure provide no evidence for the presence of such a domain in HSPC034, recently proposed as the 11th member of the human HSPB family. Also, phylogenetic analyses detect no relationship between HSPC034 and the human HSPB1–10 sequences. Arguments are provided as to why inclusion in the HSPB family of proteins like HSPC034, which resemble small heat shock proteins in being heat-inducible and having chaperone-like properties and a low monomeric mass, but are evolutionarily unrelated, is misleading and confusing.
Abbreviations
- ACD:
-
α-crystallin domain
References
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Figure S1
Alignment of sHSP and HSPC034 sequences. Names and accession numbers are: human Hs_HspB1 (P04792), Hs_HspB2 (Q16082), Hs_HspB3 (Q12988), Hs_HspB4(P02489), Hs_HspB5 (P02511), Hs_HspB6 (O14558), Hs_HspB7 (Q9UBY9), Hs_HspB8 (Q9UJY1), Hs_HSpB9 (Q9BQS6), Hs_HspB10 (Q14990); mouse Mm_HspB1 (P14602), Mm_HspB2 (Q99PR8), Mm_HspB3 (Q9QZ57), Mm_HspB4 (P24622), Mm_HspB5 (P23927), Mm_HspB6 (Q5EBG6), Mm_HspB7 (P35385), Mm_HspB8 (Q9JK92), Mm_HspB9 (Q9DAM3), Mm_HspB10 (Q61999); chicken Gg_HspB1 (Q00649), Gg_HspB2 (NP_001001527), Gg_HspB3 (XP_001231558), Gg_HspB4 (P02504), Gg_HspB5 (Q05713), Gg_HspB7 (XP_427836), Gg_HspB8 (XP_415280), Gg_HspB10(XP_418368); wheat Tae_Hsp16.9 (CAA45902); Methanococcus jannasschii Mj_Hsp16.5 (Q57733); human Hs_HSPC034 (Q9Y547), mouse Mm_HSPC034 (Q9D6H2), chicken Gg_HSPC034 (XP_422488), amphioxus Bf_HSPC0234 (XP_002207873), Trichoplax adhaerens Tad_HSPC034 (XP_002114382), and T. vaginalis Tv_HSPC034 (XP_001326124). Alignment is obtained with ClustalW v1.83 at default settings, without manual editing, to avoid any subjective influences (GIF 121 KB)
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Kappé, G., Boelens, W.C. & de Jong, W.W. Why proteins without an α-crystallin domain should not be included in the human small heat shock protein family HSPB. Cell Stress and Chaperones 15, 457–461 (2010). https://doi.org/10.1007/s12192-009-0155-4
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DOI: https://doi.org/10.1007/s12192-009-0155-4