Abstract
Foodborne illnesses are being reported everyday; thus, there is an obvious need for faster and sensitive methodologies to detect foodborne pathogens in order to assure the safeness of foods. In the present study, the detection of L. monocytogenes, Salmonella spp., and E. coli O157 was performed combining a multiplex short enrichment of 7 h in Tryptic Soy Broth, with a colorimetric LAMP-based naked-eye detection. The LAMP reaction obtained a similar sensitivity to that of real-time PCR. The methodology was evaluated in UHT, fresh and raw milk were tested, achieving a LoD95 of 1.6 CFU/25 mL for Salmonella spp. and E. coli O157 respectively without matrix interference, and for L. monocytogenes, the LoD95 was calculated to be 79.0 CFU/25 mL, showing some interference when a higher natural microflora was present in the sample. The methodology can be applied in the food industry with reliability, as the evaluation obtained a k index of 1.0 for L. monocytogenes and Salmonella spp. and 0.94 for E. coli O157.
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Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.
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Funding
SA was financed by Ph.D. grant of the Fundação para a Ciência e a Tecnologia (SFRH/BD/140396/2018). Dr. Alejandro Garrido-Maestu acknowledges funding from the Fundação para a Ciência e Tecnologia through the Scientific Employment Stimulus Program (2021.02810.CEECIND). This research was funded by Seafood Age project which was co-financed by the Interreg Atlantic Area Programme (EAPA_758/2018) through the European Development Fund (ERDF).
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Sarah Azinheiro: investigation, revision, and wrote the first draft of the manuscript.
Foteini Roumani: investigation, revision, and edition.
Marta Prado: funding acquisition, supervision, revision, and editing.
Alejandro Garrido-Maestu: analyzed the data, supervision, revision, and editing.
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Sarah Azinheiro declares that she has no conflict of interest. Foteini Roumani declares that she has no conflict of interest. Marta Prado declares that she has no conflict of interest. Alejandro Garrido-Maestu declares that he has no conflict of interest.
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Azinheiro, S., Roumani, F., Prado, M. et al. Rapid Same-Day Detection of Listeria monocytogenes, Salmonella spp., and Escherichia coli O157 by Colorimetric LAMP in Dairy Products. Food Anal. Methods 15, 2959–2971 (2022). https://doi.org/10.1007/s12161-022-02345-9
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DOI: https://doi.org/10.1007/s12161-022-02345-9