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Development of a Nanobody-Based Competitive Dot ELISA for Visual Screening of Ochratoxin A in Cereals

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Abstract

A polyvinylidene fluoride membrane-based dot immunoassay using nanobody (Nb) for rapid, qualitative, and visual detection of ochratoxin A (OTA) in cereals was developed. On the basis of optimal assay conditions, the cut-off level of this method assessed visually was 5 μg/kg for OTA, and the final results were obtained within 20 min. This method was simple with no time-consuming cleanup procedure. Good accuracy and reproducibility were obtained in recovery experiments. Results of the Nb-based dot ELISA are in agreement with the ELISA kit, except for samples that were negative for OTA presence assessed through Nb-based dot ELISA but found positive through the ELISA kit. These results indicated that the developed method could be a useful on-site screening tool for rapid detection of OTA in cereals without special instrument.

Flow chart of nanobody-based dot ELISA for OTA visual screening

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Acknowledgements

This work was financially supported by the Scientific Research Foundation of Hainan University (grant number kyqd1631) and the Academic Innovation Project for Youth Science Talents, Hainan Provincial Association for Science and Technology (grant number 201504). Partial support was provided by the Natural Science Foundation of Hainan Province (grant number 317034) and the Guangxi talent highland for preservation and deep processing research in fruits and vegetables.

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Authors and Affiliations

  1. Institute of Food Research, Hezhou University, Hezhou, 542899, China

    Zhichang Sun, Zhenhua Duan & Xing Liu

  2. College of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou, 570228, China

    Zhichang Sun, Xing Liu, Xing Deng & Zongwen Tang

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  1. Zhichang Sun
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  2. Zhenhua Duan
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  3. Xing Liu
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  4. Xing Deng
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Correspondence to Xing Liu.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Zhichang Sun declares no conflict of interest. Zhenhua Duan declares no conflict of interest. Xing Liu declares no conflict of interest. Xing Deng declares no conflict of interest. Zongwen Tang declares no conflict of interest.

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Sun, Z., Duan, Z., Liu, X. et al. Development of a Nanobody-Based Competitive Dot ELISA for Visual Screening of Ochratoxin A in Cereals. Food Anal. Methods 10, 3558–3564 (2017). https://doi.org/10.1007/s12161-017-0915-1

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  • DOI: https://doi.org/10.1007/s12161-017-0915-1

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