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Development and Validation of a LC-FD Method for the Simultaneous Determination of Eight Bisphenols in Soft Drinks

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Abstract

Bisphenol A (BPA) is a chemical widely used as a monomer in the production of polymers of plastics. It acts as an endocrine-disrupting agent and thus its contaminations of food and beverage should be carefully monitored in order to assess consumers’ risk. In this study, we propose a liquid chromatography-fluorescence detection (LC-FD)-validated method for the simultaneous determination of BPA and seven analogues, i.e., bisphenol AF, bisphenol B, bisphenol E, bisphenol F, BPA diglycidyl ether, bisphenol F diglycidyl ether, and Bisphenol M in soft drinks. A one-step solid-phase extraction (SPE) was effective at reducing the interferences, obtaining good purification of the samples, and consequently good recoveries of all analytes. The separation was obtained on a C18 column by using acetonitrile/water 55:45 (v/v) under isocratic conditions. Method validation was performed according to the European Commission Decision 2002/657/EC criteria, providing good results regarding the analytical parameters of linearity, selectivity, sensitivity, precision, recovery, decision limit (CCα), detection capability (CCβ), limit of detection (LOD), limit of quantification (LOQ), stability, and robustness. The method allows the detection of BPA and BADGE at levels much lower than their legal limits in the food, which are 0.06 and 9.00 mg kg−1, respectively.

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Acknowledgments

This work was supported by the Italian Ministry of Health (RF-2009-1536185): “Food and environmental safety: the problem of the endocrine disruptors” coordinated by D.G. Mita).

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Authors and Affiliations

  1. Dipartimento di Farmacia, Università degli Studi di Napoli Federico II, Via D. Montesano, 49, I-80131, Naples, Italy

    Giacomo Russo, Francesco Barbato & Lucia Grumetto

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  1. Giacomo Russo
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  2. Francesco Barbato
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  3. Lucia Grumetto
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Correspondence to Lucia Grumetto.

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Russo, G., Barbato, F. & Grumetto, L. Development and Validation of a LC-FD Method for the Simultaneous Determination of Eight Bisphenols in Soft Drinks. Food Anal. Methods 9, 2732–2740 (2016). https://doi.org/10.1007/s12161-016-0458-x

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  • DOI: https://doi.org/10.1007/s12161-016-0458-x

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