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Simultaneous Determination of Bisphenol A, Bisphenol F, 4-Nonylphenol, 4-n-Nonylphenol, and Octylphenol in Grease-Rich Food by Carb/PSA Solid-Phase Extraction Combined with High-Performance Liquid Chromatography Tandem Mass Spectrometry

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Abstract

A solid-phase extraction (SPE) combined with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method was developed to determine five phenols in grease-rich food (i.e., hotpot seasoning). Sample added with isotope internal standards bisphenol A-d 4 (BPA-d 4) and 4-n-nonylphenol-d 4 (4-n-NP-d 4) was dispersedly dissolved in n-hexane. After centrifugation, the supernatant was purified by Carb/PSA SPE. Then, target analytes were separated on a Waters XBridge C18 column, detected under multiple reaction monitoring (MRM) mode. The developed method was validated in terms of linearity, limit of quantitation (LOQ), recovery, and precision. Results found that linear relations were favorable in the selected concentration range of 0.5–100 μg/L for all phenols, with R 2 greater than 0.995. LOQs were between 1.0 and 2.0 μg/kg. The mean recoveries for negative sample at three spiked concentration levels were in the range of 83.5–97.1%, and the intra-day and inter-day precision were calculated between 4.7 and 12.5%. This method is accurate and sensitive and has good purifying effect, which can be applied for screening and quantitation of target phenols in grease-rich food.

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Authors and Affiliations

  1. Guangdong Agriculture Industry Business Polytechnic College, Guangzhou, China

    Zhiming Hao, Yanping Xiao & Lipeng Yuan

  2. Guangzhou Quality Supervision and Testing Institute, Guangzhou, China

    Lanlan Jiang

  3. College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China

    Weidong Bai

  4. Beijing University of Chemical Technology, Beijing, China

    Wenshan Huang

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  1. Zhiming Hao
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  2. Yanping Xiao
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  3. Lanlan Jiang
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  4. Weidong Bai
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  5. Wenshan Huang
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  6. Lipeng Yuan
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Correspondence to Weidong Bai.

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Conflict of Interest

Zhiming Hao declares that he has no conflict of interest. Yanping Xiao declares that she has no conflict of interest. Lanlan Jiang declares that she has no conflict of interest. Weidong Bai declares that he has no conflict of interest. Wenshan Huang declares that he has no conflict of interest. Lipeng Yuan declares that he has no conflict of interest.

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Hao, Z., Xiao, Y., Jiang, L. et al. Simultaneous Determination of Bisphenol A, Bisphenol F, 4-Nonylphenol, 4-n-Nonylphenol, and Octylphenol in Grease-Rich Food by Carb/PSA Solid-Phase Extraction Combined with High-Performance Liquid Chromatography Tandem Mass Spectrometry. Food Anal. Methods 11, 589–597 (2018). https://doi.org/10.1007/s12161-017-1029-5

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