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Pesticide Residue Rapid Extraction from Ginseng Tea Using a Modified Luke Method for GC–MS

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Abstract

A modified Luke rapid detection method followed by gas chromatography–mass spectrometry (GC–MS) has been developed for the simultaneous determination of 42 pesticides in ginseng tea. This method uses acetone and n-hexane solution to extract and partition pesticides. Ginseng tea samples were mixed with acetone, water and n-hexane solution, and then partitioned by vortex. After the partition, the top layer (n-hexane) was transferred into a centrifuge tube containing primary secondary amine (PSA), graphitized carbon black (GCB) and C18 for purification. And then the centrifuge supernatant was injected into GC–MS. The Luke method was applied in ginseng tea sample detection, and we confirmed that this method can easily extract various types of pesticides from ginseng tea samples. The rates of recovery for pesticides studied were satisfactory, ranging from 65.5 to 109.0 % with a relative standard deviation (RSD) of less than 12 %. The limits of quantification (LOQs) ranged between 0.5 and 11.5 μg·kg−1. The modified Luke method is quick and easy.

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    Authors and Affiliations

    1. Ginseng and Antler Products Testing Center of the Ministry of Agricultural PRC, Jilin Agricultural University, Changchun, 130118, Jilin, People’s Republic of China

      Xuanwei Xu & Yueru Li

    2. College of Resources and Environment Science, Jilin Agricultural University, Changchun, 130118, Jilin, People’s Republic of China

      Shuang Liang & Zhongbin Lu

    Authors
    1. Xuanwei Xu
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    2. Shuang Liang
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    3. Yueru Li
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    4. Zhongbin Lu
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    Correspondence to Yueru Li or Zhongbin Lu.

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    The manuscript has not been published previously (partly or in full), unless the new work concerns an expansion of previous work (please provide transparency on the reuse of material to avoid the hint of text recycling).

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    This study was funded by 2011-Z55 Analysis and Safety Warning System Form of common pesticide residue levels on ginseng in Jilin province.

    Conflict of Interest

    Xuanwei Xu declares that he has no conflict of interest. Shuang Liang declares that she has no conflict of interest. Yueru Li declares that she has no conflict of interest. Zhongbin Lu declares that he has no conflict of interest.

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    This article does not contain any studies with human participants or animals performed by any of the authors.

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    Informed consent was obtained from all individual participants included in the study.

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    Xuanwei Xu and Shuang Liang contributed equally to this work.

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    Xu, X., Liang, S., Li, Y. et al. Pesticide Residue Rapid Extraction from Ginseng Tea Using a Modified Luke Method for GC–MS. Food Anal. Methods 9, 2231–2240 (2016). https://doi.org/10.1007/s12161-016-0400-2

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    • DOI: https://doi.org/10.1007/s12161-016-0400-2

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