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Biodistribution of immunoliposome labeled with Tc-99m in tumor xenografted mice

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Abstract

Objective

Immunoliposome (PEG, GAH, liposome; PGL), consisting of F(ab′)2 fragment of monoclonal antibody, GAH and polyethyleneglycol-coated (PEGylated) liposome was provided. Immunoliposome, PGL was labeled with technetium-99m (Tc-99m) by two methods: labeling F(ab′)2 fragment with Tc-99m; Tc-99m-PGL, and entrapping Tc-99m into liposome; PGL[Tc-99m]. The objective of this study was to compare the biodistribution of Tc-99m-PGL and PGL[Tc-99m] in human gastric cancer xenografted mice.

Methods

Tc-99m-PGL, PGL[Tc-99m], and Tc-99m-entrapped liposome; Lipo[Tc-99m] were prepared. They were injected into human gastric cancer, MKN45, xenografted mice via the tail vein, and their biodistribution was studied.

Results

No marked accumulation of either PGL[Tc-99m] or Lipo[Tc-99m] was observed in the stomach. The uptake of Tc-99m-PGL by the liver, spleen, and lung was higher than that by the tumor. On the other hand, the uptake of PGL[Tc-99m] by the lung and spleen was markedly lower as compared with that of Tc-99m-PGL; the accumulation of PGL[Tc-99m] was lower in the lung and higher in the spleen as compared with that of the tumor. Although the liver uptake of PGL[Tc-99m] was markedly decreased as compared with that of Tc-99m-PGL, it was higher than the uptake of the tumor. The Tc-99m-PGL was strongly taken up by the tumor, with a high level of incorporation also seen in the stomach. These findings suggest the need for further study of the labeling stability.

Conclusions

PGL[Tc-99m] appears to show promise for high tumor uptake and retention. This is an important implication for the potential application of immunoliposomes entrapped with Re-186, instead of Tc-99m, in internal radiotherapy.

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Authors and Affiliations

  1. Department of Radiology, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Naoto Kitamura, Naoyuki Shigematsu, Tadaki Nakahara, Momoe Kanoh, Jun Hashimoto, Etsuo Kunieda & Atsushi Kubo

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  1. Naoto Kitamura
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  2. Naoyuki Shigematsu
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  3. Tadaki Nakahara
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  4. Momoe Kanoh
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  5. Jun Hashimoto
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  6. Etsuo Kunieda
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  7. Atsushi Kubo
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Correspondence to Naoto Kitamura.

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Kitamura, N., Shigematsu, N., Nakahara, T. et al. Biodistribution of immunoliposome labeled with Tc-99m in tumor xenografted mice. Ann Nucl Med 23, 149–153 (2009). https://doi.org/10.1007/s12149-008-0222-4

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  • DOI: https://doi.org/10.1007/s12149-008-0222-4

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