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Sequence specific 1H, 13C and 15N resonance assignments of a cataract-related variant G57W of human γS-crystallin

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Abstract

γS-crystallin is a major structural component of the human eye lens, which maintains its stability over the lifetime of an organism with negligible turnover. The G57W mutant of human γS-crystallin (abbreviated hereafter as γS-G57W) is associated with dominant congenital cataracts. In order to provide a structural basis for the ability of γS-G57W causing cataract, we have cloned, overexpressed, isolated and purified the protein. The 2D [15N–1H]-HSQC spectrum recorded with uniformly 13C/15N-labelled γS-G57W was highly dispersed indicating the protein to adopt an ordered conformation. In this paper, we report almost complete sequence-specific 1H, 13C and 15N resonance assignments of γS-G57W using a suite of heteronuclear 3D NMR experiments.

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Abbreviations

γS-G57W:

G57W mutant of human γS-crystallin

HSQC:

Heteronuclear single quantum correlation

NMR:

Nuclear magnetic resonance

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Acknowledgements

The facilities provided by the National Facility for High Field NMR, supported by the Department of Science and Technology (DST), Department of Biotechnology (DBT), Council of Scientific and Industrial Research (CSIR), Tata Institute of Fundamental Research (TIFR), Mumbai and the NMR Centre at Indian Institute of Chemical Technology (IICT), Hyderabad are gratefully acknowledged. KVRC acknowledges the financial support of JC Bose fellowship of DST (Govt. of India).

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Authors and Affiliations

  1. Center for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Gopanpally, Hyderabad, 500075, India

    Khandekar Jishan Bari, Shrikant Sharma & Kandala V. R. Chary

  2. Department of Chemical Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Mumbai, 400005, India

    Kandala V. R. Chary

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  1. Khandekar Jishan Bari
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  2. Shrikant Sharma
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  3. Kandala V. R. Chary
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KJB and SS have contributed equally to this work.

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Correspondence to Kandala V. R. Chary.

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Khandekar Jishan Bari and Shrikant Sharma have shared equal authorship.

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Bari, K.J., Sharma, S. & Chary, K.V.R. Sequence specific 1H, 13C and 15N resonance assignments of a cataract-related variant G57W of human γS-crystallin. Biomol NMR Assign 12, 51–55 (2018). https://doi.org/10.1007/s12104-017-9779-y

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