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Investigative Analysis of Lanthanum Oxide Nanoparticles on Elements in Bone of Wistar Rats After 30 Days of Repeated Oral Administration

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Abstract

Accumulation of lanthanum oxide nanoparticles (La2O3NPs) in the femur bone of rats after 30 days of oral administration was explored. Also, the influence of La2O3NPs on macro and trace elements in the rats’ femur bone was assessed. Inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical spectrometry (ICP OES) were used for total element determination in the bone after decomposition while laser ablation-ICP-MS (LA-ICP-MS) was used to investigate element distribution (bio-imaging) in the bone. Some differences in element concentrations in the bone between the rats treated with La2O3NPs at 1.0 mg kg−1 (T1), 10.0 mg kg−1 (T2), and 100 mg kg−1 (T3) body weight (bw) and the control rats (CTR) were observed. More differences were observed in the bone of rat treated with 10.0 mg kg−1 La2O3NPs bw. However, the highest change observed was for Mg, which concentration ranged from 5230 ± 12 μg kg−1 for the CTR group to 4130 ± 138 μg kg−1 for the T3 group. Minor changes were observed for Ba, Ca, Cr, Cu, Fe, Mg, Na, Pb, Sr, and Zn between CTR and animals treated with La2O3NPs at the different levels of concentration. It was possible to observe from LA-ICP-MS analysis that La2O3NPs were accumulated only on the surface of the bone, not deeper than about 5 μm. LA-ICP-MS allowed also to investigate the distribution of La and the other elements in a cross section of the femur bone head, where higher amounts of the elements are present at the external part of the bone. Therefore, it was demonstrated that La2O3NPs are incorporated on the surface of the bone and it has a small influence on some of the other elements evaluated.

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Acknowledgements

The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. Nr. 120230/2017-8 and Proc. nr. 306052/2017-2) for scholarships and supporting this project.

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Authors and Affiliations

  1. Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Valderi Luiz Dressler, Graciela Marine Heidrich & Vinicius Machado Neves

  2. Department of Chemical Sciences, Afe Babalola University Ado-Ekiti, P.M.B, Ado-Ekiti, 5454, Nigeria

    Oluwafemi Temitayo Ogunmodede

  3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil

    Maria Rosa Chitolina Schetinger & Vera Maria Morsch

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  1. Valderi Luiz Dressler
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Correspondence to Valderi Luiz Dressler.

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All procedures performed in studies involving animals were approved by the Animals Ethics Committee of the Federal University of Santa Maria (CEUA, protocol number 4250170317). CEUA follows the guidelines of “Conselho Nacional de Controle de Experimentação Animal (CONCEA), which is the “Brazilian Guide for the Production, Maintenance or Use of Animals for Teaching or Scientific Research Activities”.

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Dressler, V.L., Ogunmodede, O.T., Heidrich, G.M. et al. Investigative Analysis of Lanthanum Oxide Nanoparticles on Elements in Bone of Wistar Rats After 30 Days of Repeated Oral Administration. Biol Trace Elem Res 196, 153–167 (2020). https://doi.org/10.1007/s12011-019-01907-z

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