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A Novel and Effective Streptomyces sp. N2 Against Various Phytopathogenic Fungi

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Abstract

Phytopathogenic fungi would induce a variety of plant diseases, resulting in a severe reduction of agricultural output. However, the current plant disease control is mainly dependent on the environmentally and healthily hazardous chemical fungicides. Thus, the present work aimed to isolate an effective antagonistic microorganism against various soilborne phytopathogenic fungi. By dual culture with Rhizoctonia solani, a novel Streptomyces specie, Streptomyces sp. N2, was screened out from a total of 167 isolated actinomycetes, which displayed a strong inhibitory effect on R. solani (26.85 ± 1.35 mm of inhibition zone diameter). By means of macroporous resin and silica gel column chromatography coupled with preparative HPLC, an antifungal metabolite (3-methyl-3,5-amino-4-vinyl-2-pyrone, C6H7O2N) was isolated and purified from Streptomyces sp. N2. The bioassay results showed that the purified antifungal metabolite could not only possess a broad-spectrum inhibitory effect on a range of plant pathogenic fungi in vitro (e.g., R. solani, Pyricularia grisea, Fusarium oxysporum f. sp. niveum, F. oxysporum f. sp. vasinfectum, Penicillium italicum, and Colletotrichum gloeosporioides), but also had a significantly effective in vivo biocontrol efficacy on grape fruits anthracnose caused by C. gloeosporioides. Microscopic observation indicated that the antifungal metabolite from Streptomyces sp. N2 would exert its antimicrobial activity by disorganizing the cytoplasmic organelles of phytopathogenic fungi. The above results suggested that Streptomyces sp. N2 was one of promising fungicide for biocontrol of fungal plant diseases, especially due to its broad-spectrum and effective antagonist on various plant pathogens.

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Acknowledgments

This work was financially supported by the Training Program for Young Scientists of Jiangxi Provincial Department of Science and Technology (20142BCB23025), International Scientific and Technological Cooperation Projects of Jiangxi Provincial Department of Science and Technology (20141BDH80033), and Jiangxi Undergraduate Training Programs for Innovation and Entrepreneurship (201410410008).

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The authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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Authors and Affiliations

  1. Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang, 330045, China

    Bo Xu, Wei Chen, Zhi-ming Wu, Yue Long & Kun-tai Li

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  1. Bo Xu
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  2. Wei Chen
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  3. Zhi-ming Wu
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  4. Yue Long
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  5. Kun-tai Li
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Correspondence to Kun-tai Li.

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Xu, B., Chen, W., Wu, Zm. et al. A Novel and Effective Streptomyces sp. N2 Against Various Phytopathogenic Fungi. Appl Biochem Biotechnol 177, 1338–1347 (2015). https://doi.org/10.1007/s12010-015-1818-5

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  • DOI: https://doi.org/10.1007/s12010-015-1818-5

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