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Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum

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Abstract

In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 °C, and PE was thermally damaged at temperatures higher than 40 °C. In contrast, a flash irradiation for 10 s at 100 °C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary to extract them from the thylakoid membrane to a short exposure to thermal denaturation. The extraction order of the three phycobiliproteins was coherent with the structure of Pp phycobilisomes. Moreover, the absorption and fluorescence properties of MAE extracted phycobiliproteins were stable for several months after the microwave treatment. Scanning electron microscopy indicated that MAE at 100 °C induced major changes in the Pp cell morphology, including fusion of the exopolysaccharidic cell walls and cytoplasmic membranes of adjacent cells. As a conclusion, MAE is a fast and high yield process efficient to extract and pre-purify phycobiliproteins, even from microalgae containing a thick exopolysaccharidic cell wall.

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Acknowledgments

This research was financially supported by the French cancer league (Comité 17 de la Ligue Nationale contre le Cancer), European FEDER fund no. 34755-2011 (ALG post-doctoral fellowship), and CPER “Contrats de Projet Etat-Région: Poitou-Charentes” funds (project “Extraction of anticancer pigments from marine microalgae”). We are grateful to the Poitou-Charentes region for CJ’s PhD grant. We also thank the “Cancéropôle Grand Ouest, axe Valorisation des produits de la mer en cancérologie” and Dr. Hélène Montanié, Dr. Isabelle Lanneluc, and Dr. Matthieu Garnier for scientific assistance.

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Authors and Affiliations

  1. UMRi CNRS 7266 LIENSs University of La Rochelle, 17042, La Rochelle, France

    Camille Juin, Jean-René Chérouvrier, Valérie Thiéry, Anne-Laure Gagez, Nicolas Joguet & Laurent Picot

  2. IFREMER Laboratoire BRM/PBA, Rue de l’Ile d’Yeu, 44311, Nantes, France

    Jean-Baptiste Bérard, Raymond Kaas & Jean-Paul Cadoret

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  1. Camille Juin
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  2. Jean-René Chérouvrier
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  3. Valérie Thiéry
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  4. Anne-Laure Gagez
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  5. Jean-Baptiste Bérard
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  9. Laurent Picot
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Correspondence to Laurent Picot.

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Highlights

1. Phycobiliproteins are high value fluorescent microalgae pigments.

2. Most phycobiliprotein extraction processes imply the use of ionic buffers or enzymes.

3. Porphyridium purpureum phycobiliproteins can be efficiently extracted using MAE.

4. MAE gives high extraction yields and reduces extraction time.

5. Absorption and fluorescence properties of extracted pigments are not altered by MAE.

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Juin, C., Chérouvrier, JR., Thiéry, V. et al. Microwave-Assisted Extraction of Phycobiliproteins from Porphyridium purpureum . Appl Biochem Biotechnol 175, 1–15 (2015). https://doi.org/10.1007/s12010-014-1250-2

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