Abstract
Zearalenone (ZEN) is a fungal toxin produced by Fusarium that widely occurs in various types of grains and feed. It has strong reproductive toxicity and a wide range of distribution, which will impact food and feed safety. In this study, Bacillus subtilis ZENL09, which is capable of transforming ZEN was investigated. By optimizing the fermentation conditions, the supernatant of Bacillus subtilis ZENL09 fermentation can achieve about 88% degradation rate of ZEN after reacting with ZEN for 12 h. The degradation product was collected by high-performance liquid chromatography, followed by LC-Q-TOF and MS/MS analysis to identify the changes in molecular mass and compound structure. The possible structure of the degradation product was also tentatively determined. Further evaluation of the degradation products’ cytotoxicity was conducted using HepG2 cells, and the results indicated a significant reduction in toxicity compared to ZEN. Detoxification experiments on corn steep liquor showed that the fermentation supernatant of Bacillus subtilis degraded 87% of ZEN within 12 h, which further validates its potential application for detoxification in food and agricultural products.
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This work was supported by key research and development program of Hubei Province (No. 2022BBA0053).
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MX: conceptualization, methodology, investigation, writing - original draft. JC: conceptualization. PL: validation, investigation, resources. HZ: formal analysis. ML: formal analysis, resources. QD: formal analysis, resources.
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Xiang, M., Liu, P., Zhang, H. et al. In Vitro Degradation of Zearalenone by Culture Supernatant of Bacillus subtilis. Food Bioprocess Technol (2023). https://doi.org/10.1007/s11947-023-03254-3
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DOI: https://doi.org/10.1007/s11947-023-03254-3