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Lipid Emulsions Containing Medium Chain Triacylglycerols Blunt Bradykinin-Induced Endothelium-Dependent Relaxation in Porcine Coronary Artery Rings

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Lipids

Abstract

Lipid emulsions for parenteral nutrition are used to provide calories and essential fatty acids for patients. They have been associated with hypertriglyceridemia, hypercholesterolemia, and metabolic stress, which may promote the development of endothelial dysfunction in patients. The aim of the present study was to determine whether five different industrial lipid emulsions may affect the endothelial function of coronary arteries. Porcine coronary artery rings were incubated with lipid emulsions 0.5, 1, or 2% (v/v) for 30 min before the determination of vascular reactivity in organ chambers and the level of oxidative stress using electron paramagnetic resonance. Incubation of coronary artery rings with either Lipidem®, Medialipid® containing long- and medium-chain triacylglycerols (LCT/MCT), or SMOFlipid® containing LCT, MCT, omega-9, and -3, significantly reduced the bradykinin-induced endothelium-dependent relaxation, affecting both the nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) components, whereas, Intralipid® containing LCT (soybean oil) and ClinOleic® containing LCT (soybean and olive oil) did not have such an effect. The endothelial dysfunction induced by Lipidem® was significantly improved by indomethacin, a cyclooxygenase (COX) inhibitor, inhibitors of oxidative stress (N-acetylcysteine, superoxide dismutase, catalase) and transition metal chelating agents (neocuproine, tetrathiomolybdate, deferoxamine and l-histidine). Lipidem® significantly increased the arterial level of oxidative stress. The present findings indicate that lipid emulsions containing LCT/MCT induce endothelial dysfunction in coronary artery rings by blunting both NO- and EDH-mediated relaxations. The Lipidem®-induced endothelial dysfunction is associated with increased vascular oxidative stress and the formation of COX-derived vasoconstrictor prostanoids.

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Abbreviations

DHA:

Docosahexaenoic acid (22:6n-3)

EDH:

Endothelium-dependent hyperpolarization

EPA:

Eicosapentaenoic acid (20:5n-3)

KCN:

Potassium cyanide

LCT:

Long chain triacylglycerols

MCT:

Medium chain triacylglycerols

MnTMPyP:

Mn(III) Tetrakis(1-methyl-4 pyridyl) porphyrin

NAC:

N-acetylcysteine

NO:

Nitric oxide

PUFA:

Polyunsaturated fatty acid

ROS:

Reactive oxygen species

VAS-2870:

3-Benzyl-7-(2-benzoxazolyl) thio-1,2,3-triazolo (4,5-d) pyrimidine

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Authors and Affiliations

  1. UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France

    Said Amissi, Mélanie Burban, Sherzad K. Rashid, Antonio J. León-González, Cyril Auger, Florence Toti & Valérie B. Schini-Kerth

  2. EA 7293, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Faculté de Médecine, Université de Strasbourg, Strasbourg, France

    Julie Boisramé-Helms & Ferhat Meziani

  3. Service de Réanimation Médicale, Nouvel Hôpital Civil, Hôpitaux Universitaires de Strasbourg, Strasbourg, France

    Julie Boisramé-Helms & Ferhat Meziani

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  1. Said Amissi
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  2. Julie Boisramé-Helms
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  3. Mélanie Burban
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  4. Sherzad K. Rashid
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  6. Cyril Auger
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  7. Florence Toti
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  9. Valérie B. Schini-Kerth
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Correspondence to Valérie B. Schini-Kerth.

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Amissi, S., Boisramé-Helms, J., Burban, M. et al. Lipid Emulsions Containing Medium Chain Triacylglycerols Blunt Bradykinin-Induced Endothelium-Dependent Relaxation in Porcine Coronary Artery Rings. Lipids 52, 235–243 (2017). https://doi.org/10.1007/s11745-016-4225-y

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