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Stanniocalcin 1 Enhances Carbon Flux from Glucose to Lipids in White Retroperitoneal Adipose Tissue in the Fed Rat

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Lipids

Abstract

The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of 14C from glucose into lipids in the rat WRAT. The increase in lipogenesis capacity supports the hypothesis that the activity of the glycerol-3-phosphate-generating pathway (glycolysis) from glucose is regulated by hSTC-1. The effect of hSTC-1 on de novo fatty acid synthesis and on glucose oxidation in WRAT is supported by an 85 % increase in 14CO2 production from 14C-glucose. The incubation of WRAT in the presence of hSTC-1 maintained the ADP/ATP ratio close to the control group. The presence of hSTC-1 in the incubation medium did not inhibit the lipolytic effect of epinephrine. In conclusion, hSTC-1 is one of the hormonal factors that control glucose metabolism in WRAT in the fed state.

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Abbreviations

G3P:

Glycerol 3 Phosphate

hSTC-1:

Human Stanniocalcin 1

KH:

Krebs Henseleit

NADPH:

Nicotinamide adenine dinucleotide phosphate

TAG:

Triacylglycerols

WAT:

White adipose tissue

WRAT:

White retroperitoneal adipose tissue

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Acknowledgments

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. RSMS and LCK are PQ-CNPq fellows, and AGC and JFAM are CNPq fellows, and VS is a PNPD-CAPES fellow. The experiments were performed according to the current Brazilian laws.

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Authors and Affiliations

  1. Department of Physiology, Institute of Health Sciences, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, RS, Brazil

    Aline G. Cozer, Márcia Trapp, Claudia Vieira Marques, Tiago L. Martins, Jorge Felipe A. Model, Vanessa Schein, Luiz Carlos Kucharski & Roselis S. M. Da Silva

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  1. Aline G. Cozer
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  2. Márcia Trapp
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  3. Claudia Vieira Marques
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  5. Jorge Felipe A. Model
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  6. Vanessa Schein
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  7. Luiz Carlos Kucharski
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  8. Roselis S. M. Da Silva
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Correspondence to Aline G. Cozer.

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Cozer, A.G., Trapp, M., Vieira Marques, C. et al. Stanniocalcin 1 Enhances Carbon Flux from Glucose to Lipids in White Retroperitoneal Adipose Tissue in the Fed Rat. Lipids 51, 1303–1307 (2016). https://doi.org/10.1007/s11745-016-4202-5

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  • DOI: https://doi.org/10.1007/s11745-016-4202-5

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