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Antimicrobial power of biosynthesized Ag nanoparticles using refined Ginkgo biloba leaf extracts

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Abstract

Silver nanoparticles (Ag NPs), relative to existing antibacterial agents, are more effective, less toxic and more economical, and have shown enormous potential for the nanomedicine application. In this work, we report a ‘green’ method for the rapid and efficient synthesis of Ag NPs using Ginkgo biloba extracts as reducing agent and capping agent. The properties of Ag NPs against fungi and bacteria were investigated. The results showed that the Ginkgo biloba extracts are crucial for the preparation of uniform and monodispersed Ag NPs. The prepared Ag NPs exhibited remarkable antibacterial activities. The minimum inhibitory concentrations of Ag NPs for Escherichia coli and Pseudomonas aeruginosa were 0.044 and 0.088 µg·mL−1, respectively. Moreover, Ag NPs exhibited excellent bactericidal performance against MDR-Pseudomonas aeruginosa. It was found that the effect of the antibacterial activity of Ag NPs on Escherichia coli and Staphylococcus aureus was tightly related to the reactive oxygen species accumulation. This research provides guidelines for the efficient green synthesis of Ag NPs and its antibacterial applications.

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Acknowledgements

The authors acknowledge financial supports by the National Natural Science Foundation of China (Grant Nos. 51871196 and 81960322), the Yunnan Fundamental Research Projects (Grant No. 202001BB050046), the Major Science and Technology Project of Precious Metal Materials Genetic Engineering in Yunnan Province (Grant No. 202002AB080001), the joint fund of Yunnan University and Science & Technology Department of Yunnan Province (Grant No. 2019FY003013), and the Medical Reserve Personnel Training Program of Yunnan Provincial Health Commission (Grant No. H-2018097).

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Author notes

  1. W.Z. and D.H. contributed equally to this work.

Authors and Affiliations

  1. National Center for International Research on Photoelectric and Energy Materials, Advanced Computing Center, Materials Genome Institute, School of Materials and Energy, Yunnan University, Kunming, 650091, China

    Wenbo Zhuang, Dafeng Hu, Xudong Zhang, Kai Xiong, Yong Mao, Peng Yang & Yanfen Wan

  2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

    Xiao Ding

  3. Department of Nuclear Medicine, Yunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University, Kunming, 650118, China

    Jian Lu & Chao Liu

Authors
  1. Wenbo Zhuang
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  2. Dafeng Hu
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  3. Xudong Zhang
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  4. Kai Xiong
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  5. Xiao Ding
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  7. Yong Mao
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  8. Peng Yang
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  9. Chao Liu
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  10. Yanfen Wan
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Correspondence to Peng Yang, Chao Liu or Yanfen Wan.

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Zhuang, W., Hu, D., Zhang, X. et al. Antimicrobial power of biosynthesized Ag nanoparticles using refined Ginkgo biloba leaf extracts. Front. Mater. Sci. 16, 220594 (2022). https://doi.org/10.1007/s11706-022-0594-8

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  • DOI: https://doi.org/10.1007/s11706-022-0594-8

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