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Morphological and physiological responses of tara (Caesalpinia spinosa (Mol.) O. Kuntz) microshoots to ventilation and sucrose treatments

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Abstract

One of the main problems of in vitro plant tissue culture is the poor ventilation in the culture vessels. This leads to morphological and physiological anomalies in regenerated in vitro plants, a crucial issue in woody species, such as Caesalpinia spinosa (Mol.) O. Kuntz, which is a legominous tree or thorny shrub of the Andes, adequate photoautotrophic and photomixotrophic in vitro rooting systems could eliminate these problems. Therefore, the purpose of the present study was to evaluate photoautotrophic and photomixotrophic treatments to optimize the in vitro rooting of C. spinosa microshoots. Micropropagated shoots were cultured in vessels sealed with a polypropylene lid with 0, 1, or 3 ventilation holes covered with a 0.45-μm adhesive polypropylene microfilter. The vessels contained MS salts with or without 30 g L−1 sucrose or, alternatively, a porous substrate (PRO-MIX®), which facilitated rooting and produced greater fresh and dry weights, a higher concentration of photosynthetic pigments and phenolic compounds, and more developed leaves. The optimal performance and higher survival was obtained by using PRO-MIX® with three filters for increased ventilation. For treatments in agar, only the one with sucrose and highly ventilated (three filters) reached a performance approaching that with PRO-MIX®. To date, the presented results have not been reported in scientific literature for C. spinosa.

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Acknowledgments

We acknowledge the international scientific network BIOALI-CYTED, which contributed to the presented insights and to establish coauthor contacts.

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Authors and Affiliations

  1. Escuela Superior Politécnica de Chimborazo, Panamericana sur km 11/2, Riobamba, Chimborazo, Ecuador

    Jenny Elizabeth Núñez-Ramos

  2. Instituto Nacional de Investigaciones Agropecuarias, INIAP, Av. Eloy Alfaro N30-350 y Amazonas, Quito, Ecuador

    Elisa Quiala, Saúl Mestanza & Leidy Sarmiento

  3. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní Km 5 ½, Santa Clara, Villa Clara, Cuba

    Laisyn Posada

  4. Faculty of Science & Technology, University of Belize, Hummingbird Avenue, Belmopan, Cayo District, Belize

    Dion Daniels

  5. Centro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Quito, Ecuador

    Carlos R. Arroyo & Karla Vizuete

  6. Biología Celular y Molecular de Plantas / Biotecnología Industrial y Bioproductos, Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Quito, Ecuador

    Blanca Naranjo & Carlos Noceda

  7. Facultad de Ciencias de la Ingeniería, Universidad Estatal de Milagro, Milagro, Ecuador

    Carlos Noceda

  8. Functional Cell Reprogramming and Organism Plasticity (FunCROP), University of Évora, Évora, Portugal

    Carlos Noceda

  9. Estación Territorial de Investigaciones de la Caña de Azúcar (ETICA centro-Villa Clara), Instituto de Investigaciones de la Caña de Azúcar (INICA), Autopista Nacional km 246, Ranchuelo, Villa Clara, Cuba

    Rafael Gómez-Kosky

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  1. Jenny Elizabeth Núñez-Ramos
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  2. Elisa Quiala
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Correspondence to Jenny Elizabeth Núñez-Ramos.

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Núñez-Ramos, J.E., Quiala, E., Posada, L. et al. Morphological and physiological responses of tara (Caesalpinia spinosa (Mol.) O. Kuntz) microshoots to ventilation and sucrose treatments. In Vitro Cell.Dev.Biol.-Plant 57, 1–14 (2021). https://doi.org/10.1007/s11627-020-10104-w

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