Abstract
We established a protocol for the in vitro propagation of Baccharis conferta Kunth. This plant is used to treat gastrointestinal problems, cramps, pain, respiratory problems, and insect bites. A high rate of shoot multiplication was obtained from nodal segments on Murashige and Skoog (MS) culture medium. The shoots regenerated roots without exogenous plant growth regulators (PGRs). All explants of wild leaves on MS medium containing 5 μM of thidiazuron (TDZ) produced friable callus. An organogenic response was achieved after 3 wk of culture when callus segments were transferred to MS medium containing a combination of plant growth regulators (PGRs): either (i) 5 μM indole butyric acid (IBA) + 5 μM kinetin (KIN) or (ii) 0.5 μM IBA + 1.10 μM benzylaminopurine (BAP). The morphogenetic responses of callus were characterized by scanning electron microscopy. Shoots regenerated from callus and formed roots on MS medium without PGRs. The micropropagated plantlets and the organogenic callus showed similar chemical profiles in HPLC-mass spectrometry analyses. The main compounds present in the cultures were caffeoylquinic acids. Only plantlets contained small amounts of triterpenes (erythrodiol and ursolic acid). These findings will be useful for the micropropagation of this important native resource, and for further studies on its biology.
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Acknowledgments
We thank Jennifer Smith, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.
Funding
This research was financed by the Secretaría de Investigación y Posgrado of Instituto Politécnico Nacional (grant 20180034) and by the Consejo Nacional de Ciencia y Tecnología (CONACyT-México) (Grant CB-2013-01-220007). ALLP is indebted to CONACyT for an awarded fellowship and to the Programa Institucional de Formación de Investigadores (BEIFI-IPN).
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This work is based on the MSc thesis of ALLP under the supervision of GTT and JLTE. Experiments were performed by ALLP. GSM advised on in vitro culture. VMP and ARLL performed phytochemical analysis. GTT and JLTE designed the study and wrote the manuscript. All authors read and approved the final version of this paper.
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Editor: Yong Eui Choi
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Leyva-Peralta, A.L., Salcedo-Morales, G., Medina-Pérez, V. et al. Morphogenesis and in vitro production of caffeoylquinic and caffeic acids in Baccharis conferta Kunth. In Vitro Cell.Dev.Biol.-Plant 55, 581–589 (2019). https://doi.org/10.1007/s11627-019-09977-3
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DOI: https://doi.org/10.1007/s11627-019-09977-3