Abstract
Among citrus fruits, Citrus × latifolia (Yu. Tanaka) Yu. Tanaka stands out given its diverse uses, and there is a great demand for plants of high phytosanitary and genetic quality. In the present study, the in vitro micropropagation process of C. latifolia was studied in RITA®-type bioreactor, semisolid medium, and partial immersion with liquid culture medium. Explants that were 2 mo of age were used and apices of 0.5 cm were extracted and placed in each culture system. In all the systems, MS (Murashige and Skoog) culture medium was used, supplemented with 6-benzylaminopurine (BAP) and kinetin (KIN). After 30 d, the number of shoots, the size of the shoots, the number of leaves, and the depletion of nutrients from the culture medium were analyzed. With the data obtained, ANOVA and Tukey’s mean comparison tests were performed. The RITA® bioreactors, with the addition of 40 mL of medium per explant in a 4-h-interval immersion, facilitated the production of the greatest number of shoots compared to the other culture systems, and also resulted in the highest levels in medium nutrient depletion.
Similar content being viewed by others
References
Ahmadian M, Babaei A, Shokri S, Hessami S (2017) Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid culture. J Genet Eng Biotechnol 15:309–315
Akdemir H, Süzerer V, Onay A, Tilkat E, Ersali Y, Çiftçi YO (2014) Micropropagation of the pistachio and its rootstocks by temporary immersion system. Plant Cell Tissue Organ Cult 117:65–76
Alcántar GG, Sandoval MV (1999) Manual de análisis químico de tejido vegetal. Publicación especial. Núm. 10. SMCS. Chapingo, Texcoco, Estado de México. 150 p
Bassan MM, Alves Mourao FA, Miyata LY, Januzzi-Mendes BM (2011) In vitro organogenesis from internodal segments of adult sweet orange plants. Pesq Agropec Bras 46:672–674
Bordas M, Torrents J, Navarro L (2015) Micropropagation for evaluation of new Citrus somatic hybrid rootstocks. Acta Hortic 1065:329–334. https://doi.org/10.17660/ActaHortic.2015.1065.39
Broadley M, Brown P, Cakmak I, Rengel Z, Zhao F (2012) In: Marschner P (ed) Function of nutrients: micronutrients, Marschner’s mineral nutrition of higher plants. Academic Press, Burlington, pp 191–248
Cardoso JC, Abdelgalel AM, Chiancone B, Latado RR, Lain O, Testolin R, Germanà MA (2016) Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes. Plant Biosyst 150:304–312. https://doi.org/10.1080/11263504.2014.987847
Cervera M, Navarro A, Navarro L, Peña L (2008) Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration. Tree Physiol 28:55–66
Clostre G, Suni M (2007) Efecto del nitrógeno, fósforo y potasio del medio de cultivo en el rendimiento y valor nutritivo de Lemna gibba L. (Lemnaceae). Rev Peru Biol 13:231–235
Curtis IS, Mirkov TE (2012) Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin. Plant Cell Tissue Organ Cult 108:345–352
El-Hawaz R, Park D, Bridges WC, Adelberg J (2016) Optimizing in vitro mineral nutrition and plant density increases greenhouse growth of Curcuma longa L. during acclimatization. Plant Cell Tissue Organ Cult 126:33–42
El-Sherif NA (2017) Impact of plant tissue culture on agricultural sustainability. In: Barceló D, Kostianoy AG (eds) The handbook of environmental chemistry. Springer, Berlin
Food and Agricultural Organization (2017) Citrus fruit - fresh and processed statistical bulletin 2016. Food and agriculture organization of the united nations, Rome
Gao M, Jiang W, Wei S, Lin Z, Cai B, Yang L, Luo C, He X, Tan J, Chen L (2015) High-efficiency propagation of Chinese water chestnut [Eleocharis dulcis (Burm.f.) Trin. ex Hensch] using a temporary immersion bioreactor system. Plant Cell Tissue Organ Cult 121:761–772
Gentile A, Deng Z, LaMalfa S, Distefano G, Domina F, Vitale A, Polizzi G, Lorito M, Tribulato E (2007) Enhanced resistance to Phoma tracheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene. Plant Breed 126:146–151
Georgiev V, Schumann A, Pavlov A, Bley T (2014) Temporary immersion systems in plant biotechnology. Eng Life Sci 14:607–621. https://doi.org/10.1002/elsc.201300166
Jaiswal DK, Prakash Verma J, Prakash S, Meena VS, Meena RS (2016) Potassium as an important plant nutrient in sustainable agriculture: a state of the art. In: Meena VS, Maurya BR, Verma JP, Meena RS (eds) Potassium solubilizing microorganisms for sustainable agriculture. Springer, New Delhi, pp 21–29
Jin S, Park J, Park S, Lee D, Yun S (2017) Production of citrus plants from ovule cell culture and verification of CTV-free plants. Hortic Sci Technol 35:121–130
Kintzios S, Stavropoulou E, Skamneli S (2004) Accumulation of selected macronutrients and carbohydrates in melon tissue cultures: association with pathways of in vitro dedifferentiation and differentiation (organogenesis, somatic embryogenesis). Plant Sci 167:655–664
Krapp A (2015) Plant nitrogen assimilation and its regulation: a complex puzzle with missing pieces. Plant Biol 25:115–122
Mosqueda-Frometa O, Escalona-Morgado MM, Teixeira da Silva JA, Pina-Morgado DT, Daquinta-Gradaille MA (2017) In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor. Plant Cell Tissue Organ Cult 129:543–551
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Navarro-García N, Morte A, Pérez-Tornero O (2016) In vitro adventitious organogenesis and histological characterization from mature nodal explants of Citrus limon. In Vitro Cell Dev Biol-Plant 52:161–173
Pérez-Tornero O, Tallón CI, Porras I (2010) An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments. Plant Cell Tissue Organ Cult 100:263–271
Posada-Pérez L, Montesinos YP, Guerra DG, Daniels D, Gómez-Kosky R (2017) Complete germination of papaya (Carica papaya L. cv. ‘Maradol Roja’) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semisolid culture medium. In Vitro Cell Dev Biol-Plant 53:505–513
Ptak A, Simlat M, Kwiecień M, Laurain-Mattar D (2013) Leucojum aestivum plants propagated in vitro bioreactor culture and on solid media containing cytokinins. Eng Life Sci 13:261–270
Ramfula D, Tarnusb E, Aruomac OI, Bourdonb E, Bahorund T (2011) Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Res Int 44:2088–2099
Ramírez-Mosqueda MA, Iglesias-Andreu LG (2016) Evaluation of different temporary immersion systems (BIT®, BIG, and RITA®) in the micropropagation of Vanilla planifolia Jacks. In Vitro Cell Dev Biol-Plant 52:154–160
Schachtman DP, Reid RJ, Ayling SM (1998) Phosphorus uptake by plants: from soil to cell. Plant Physiol 116:447–453
Servicio de Información Agroalimentaria y Pesquera (2016) http://www.siap.sagarpa.gob.mx/ Accessed 20-03-2018
Tallón CI, Córdoba F, Porras I, Pérez-Tornero O (2015) Efficient in vitro propagation and rooting of adult explants of citrus rootstocks. Acta Hortic 1065:649–656. https://doi.org/10.17660/ActaHortic.2015.1065.81
Tallón CI, Porras I, Pérez-Tornero O (2013) High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium. In Vitro Cell Dev Biol-Plant 49:145–155
Werner S, Maschke RW, Eibl D, Eibl R (2017) Bioreactor technology for sustainable production of plant cell-derived products. In: Pavlov A, Bley T (eds) Bioprocessing of plant in vitro systems, Reference series in phytochemistry. Springer, Basel, pp 1–20
White PJ (2015) Calcium. In: Barker A, Pilbeam D (ed) Handbook of plant nutrition. CRC Press, Boca Raton, pp 165–189
Acknowledgements
The authors wish to thank the Comité Ejecutivo Nacional Sistema Producto Limón Persa A. C. Cuitláhuac, Veracruz, México, for their support.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Editor: Randall Niedz
Rights and permissions
About this article
Cite this article
Bulbarela-Marini, J.E., Gómez-Merino, F., Galindo-Tovar, M.E. et al. The in vitro propagation system of Citrus × latifolia (Yu. Tanaka) Yu. Tanaka (Rutaceae) affects the growth and depletion of nutriments. In Vitro Cell.Dev.Biol.-Plant 55, 290–295 (2019). https://doi.org/10.1007/s11627-019-09976-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-019-09976-4