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Cultivation of fractionated cells from a bioactive-alkaloid-bearing marine sponge Axinella sp.

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Abstract

Sponges are among the most primitive multicellular organisms and well-known as a major source of marine natural products. Cultivation of sponge cells has long been an attractive topic due to the prominent evolutionary and cytological significance of sponges and as a potential approach to supply sponge-derived compounds. Sponge cell culture is carried out through culturing organized cell aggregates called ‘primmorphs.’ Most research culturing sponge cells has used unfractionated cells to develop primmorphs. In the current study, a tropical marine sponge Axinella sp., which contains the bioactive alkaloids, debromohymenialdisine (DBH), and hymenialdisine (HD), was used to obtain fractionated cells and the corresponding primmorphs. These alkaloids, DBH and HD, reportedly show pharmacological activities for treating osteoarthritis and Alzheimer’s disease. Three different cell fractions were obtained, including enriched spherulous cells, large mesohyl cells, and small epithelial cells. These cell fractions were cultivated separately, forming aggregates that later developed into different kinds of primmorphs. The three kinds of primmorphs obtained were compared as regards to appearance, morphogenesis, and cellular composition. Additionally, the amount of alkaloid in the primmorphs-culture system was examined over a 30-d culturing period. During the culturing of enriched spherulous cells and developed primmorphs, the total amount of alkaloid declined notably. In addition, the speculation of alkaloid secretion and some phenomena that occurred during cell culturing are discussed.

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Funding

This research was funded by the China Scholarship Council (201908210162), the ‘Hi-Tech Research and Development Program of China’ (2006AA09Z435), and the National Natural Science Foundation of China (31801954).

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Authors and Affiliations

  1. College of Food Science and Engineering, Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian, China

    Yuefan Song

  2. Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

    Yuefan Song, Fuming Zhang & Robert J. Linhardt

  3. Dalian Environmental Monitoring Center, Dalian, China

    Yi Qu

  4. State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China

    Xupeng Cao

  5. Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Adelaide, South Australia, Australia

    Wei Zhang

  6. Center for Marine Drugs, State Key Laboratory of Oncogene and Related Genes, Department of Pharmacy, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

    Qi Yang

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  1. Yuefan Song
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Correspondence to Yuefan Song.

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Editor: Tetsuji Okamoto

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Song, Y., Qu, Y., Cao, X. et al. Cultivation of fractionated cells from a bioactive-alkaloid-bearing marine sponge Axinella sp.. In Vitro Cell.Dev.Biol.-Animal 57, 539–549 (2021). https://doi.org/10.1007/s11626-021-00578-2

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