Skip to main content
SpringerLink
Log in

Pseudopestalotiopsis ignota and Ps. camelliae spp. nov. associated with grey blight disease of tea in China

  • Original Article
  • Published:
Mycological Progress Aims and scope Submit manuscript

Abstract

We combined morphological and combined ITS, TUB and TEF sequence data to infer the phylogeny of the recently introduced genus Pseudopestalotiopsis. Two new species from China, Ps. camelliae from blighted leaves of Camellia sinensis and Ps. ignota from an undetermined host in China, are introduced with illustrated accounts and compared with similar taxa. Grey blight disease of tea appears to be caused by a group of fungi that constitutes a species complex. A new combination is proposed to emend the monophyly of Pseudopestalotiopsis and a species comparison is provided for taxa in the genus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Agnihothrudu V (1964) A world list of fungi reported on tea. J Madras Univ 34:155–271

    Google Scholar 

  • Douanla-Meli C, Langer E (2009) Pestalotiopsis theae (Ascomycota, Amphisphaeriaceae) on seeds of Diospyros crassiflora (Ebenaceae). Mycotaxon 107:441–448

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    CAS  PubMed  PubMed Central  Google Scholar 

  • Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis (Palmae) using morphological and molecular techniques. New Phytol 147:617–630. doi:10.1046/j.1469-8137.2000.00716.x

    Article  CAS  Google Scholar 

  • Horikawa T (1986) Yield loss of new tea shoots due to grey blight caused by Pestalotia longiseta Spegazzini. Bull Shizuoka Tea Exp Stn 12:1–8

    Google Scholar 

  • Hu HL, Jeewon R, Zhou DQ, Zhou TX, Hyde KD (2007) Phylogenetic diversity of endophytic Pestalotiopsis species in Pinus armandii and Ribes spp.: evidence from rDNA and β- tubulin gene phylogenies. Fungal Divers 24:1–22

    CAS  Google Scholar 

  • Index Fungorum (2016) http://www.indexfungorum.org/Names/Names.asp

  • Jayasiri SC, Hyde KD, Bhat DJ et al (2015) The Faces of Fungi database: fungal names linked with morphology, molecular and human attributes. Fungal Divers 74:3–18. doi:10.1007/s13225-015-0351-8

    Article  Google Scholar 

  • Joshi SD, Sanjay R, Baby UI, Mandal AKA (2009) Molecular characterization of Pestalotiopsis spp. associated with tea (Camellia sinensis) in southern India using RAPD and ISSR markers. Indian J Biotechnol 8:377–383

    CAS  Google Scholar 

  • Kishino H, Hasegawa M (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data. J Mol Evol 29:170–179

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Stecher G, Peterson D (2012) MEGA-CC: computing core of molecular evolutionary genetics analysis program for automated and iterative data analysis. Bioinformatics 28:2685–2686. doi:10.1093/bioinformatics/bts507

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lateef AA, Sepiah M, Bolhassan MH (2015) Description of Pseudopestalotiopsis kubahensis sp. nov., a new species of microfungi from Kubah National Park, Sarawak, Malaysia. Curr Res Environ Appl Mycol 5:376–381. doi:10.5943/cream/5/4/8

    Google Scholar 

  • Maharachchikumbura SSN, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD (2011) Pestalotiopsis – morphology, phylogeny, biochemistry and diversity. Fungal Divers 50:167–187. doi:10.1007/s13225-011-0125-x

    Article  Google Scholar 

  • Maharachchikumbura SSN, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EHC, Bahkali AH, Hyde KD (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Divers 56:95–129. doi:10.1007/s13225-012-0198-1

    Article  Google Scholar 

  • Maharachchikumbura SSN, Chukeatirote E, Guo LD, Crous PW, McKenzie EHC, Hyde KD (2013a) Pestalotiopsis species associated with Camellia sinensis (tea). Mycotaxon 123:47–61. doi:10.5248/123.47

    Article  Google Scholar 

  • Maharachchikumbura SSN, Guo LD, Chukeatirote E, Hyde KD (2013b) Improving the backbone tree for the genus Pestalotiopsis; addition of P. steyaertii and P. magna sp. nov. Mycol Prog 13:617–624. doi:10.1007/s11557-013-0944-0

    Article  Google Scholar 

  • Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW (2014) Pestalotiopsis revisited. Stud Mycol 79:121–186. doi:10.1016/j.simyco.2014.09.005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Muraleedharan N, Chen ZM (1997) Pests and diseases of tea and their management. J Plant Crop 25:15–43

    Google Scholar 

  • O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116. doi:10.1006/mpev.1996.0376

    Article  PubMed  Google Scholar 

  • Rayner RW (1970) A mycological colour chart. Commonwealth Mycological Institute, Kew and British Mycological Society, Kew, UK

    Google Scholar 

  • Rehner SA (2001) Primers for Elongation Factor 1-alpha (EF1-alpha) http://ocid.nacse.org/research/deephyphae/EF1primer.pdf

  • Senanayake IC, Maharachchikumbura SSN, Hyde KD et al (2015) Towards unraveling relationships in Xylariomycetidae (Sordariomycetes). Fungal Divers 73:1–85. doi:10.1007/s13225-015-0340-y

    Article  Google Scholar 

  • Silvestro D, Michalak I (2011) raxmlGUI: a graphical front-end for RAxML. Org Divers Evol 12:335–337. doi:10.1007/s13127-011-0056-0

    Article  Google Scholar 

  • Song Y, Tangthirasunun N, Maharachchikumbura SSN, Jiang Y, Xu J, Hyde KD, Wang Y (2014) Novel Pestalotiopsis species from Thailand point to the rich undiscovered diversity of this chemically creative genus. Cryptogam Mycol 35:139–149. doi:10.7872/crym.v35.iss2.2014.139

    Article  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer, Sunderland

  • Tejesvi MV, Nalini MS, Mahesh B, Prakash HS, Kini KR, Shetty HS, Subbiah V (2007) New hopes from endophytic fungal secondary metabolites. Bol Soc Quím Méx 1:19–26

    Google Scholar 

  • Wei JG, Xu T, Guo LD, Liu AR, Zhang Y, Pan XH (2007) Endophytic Pestalotiopsis species associated with plants of Podocarpaceae, Theaceae and Taxaceae in southern China. Fungal Divers 24:55–74

    CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J W (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315–322 In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. . Academic, New York

  • Xu J, Yang X, Lin Q (2014) Chemistry and biology of Pestalotiopsis derived natural products. Fungal Divers 66:37–68. doi:10.1007/s13225-014-0288-3

    Article  Google Scholar 

Download references

Acknowledgment

The authors would like to thank the featured microbial resources and diversity investigation in Southwest Karst area (2014FY120100).

Author information

Authors and Affiliations

  1. Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Xiaohe District, Guiyang City, Guizhou Province, 550006, People’s Republic of China

    Sajeewa S.N. Maharachchikumbura & Zuo-Yi Liu

  2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Liang-Dong Guo

  3. Institute of Excellence in Fungal Research, Mae Fah Luang University, Tasud, Muang, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

  4. School of Science, Mae Fah Luang University, Tasud, Muang, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

Authors
  1. Sajeewa S.N. Maharachchikumbura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liang-Dong Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zuo-Yi Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kevin D. Hyde
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zuo-Yi Liu.

Additional information

Section Editor: Roland Kirschner

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Maharachchikumbura, S.S., Guo, LD., Liu, ZY. et al. Pseudopestalotiopsis ignota and Ps. camelliae spp. nov. associated with grey blight disease of tea in China. Mycol Progress 15, 22 (2016). https://doi.org/10.1007/s11557-016-1162-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11557-016-1162-3

Keywords

Search

Navigation