Abstract
Troponin I, as a cardiac-specific biomarker, is a very valuable tool in the identification of acute coronary syndromes. In this work, an economical technique for easy and sensitive diagnosis of cardiac troponin I (cTnI) was developed nanoprobe on the basis of graphene oxide quenching of the fluorescence of bovine serum albumin–capped fluorescent nickel (BSA@NiNCs) nanoclusters. The developed nanoprobe nickel nanocluster exhibited superparamagnetism. The nanoprobe showed a fluorescence turn-on behavior toward incremental addition of in cTnI, with a detection limit of 0.012 ng/mL. We successfully used the proposed sensing technique for cTnI sensing in serum samples.
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Acknowledgements
The authors thank the Head of the Chemistry Department at the University of Kerala in Thiruvananthapuram, Kerala, for his support and the use of the department’s laboratory and instruments and University of Kerala and CLIF, University of Kerala, for the sophisticated instrumental analysis offered. The authors Ali Ibrahim Shkhair and Geneva Indongo thank the Government of India for the Indian Council for Cultural Relationship (ICCR) scholarship.
Funding
This research was funded by the Indian Council for Cultural Relations ICCR with Ref. No. RBI0072494658807.
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All authors contributed to the study. The author, Anju S Madanan, conceived the idea of the work. Ali Ibrahim Shkhair performed design, material preparation, data collection, and analysis. Ali Ibrahim Shkhair wrote the original manuscript draft, and Susan Varghese, Merin K. Abraham, Geneva Indongo, Greeshma Rajeevan, and Arathy B. K and Sara Muneer Abbas commented on previous versions. All authors read and approved the final manuscript.
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Shkhair, A.I., Madanan, A.S., Varghese, S. et al. Nickel Nanocluster as a Fluorescent Probe for the Non-enzymatic Detection of Cardiac Troponin I. Plasmonics (2024). https://doi.org/10.1007/s11468-024-02311-7
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DOI: https://doi.org/10.1007/s11468-024-02311-7