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Metabolic adaptation of fungal strains in response to contamination by polychlorinated biphenyls

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Abstract

Polychlorinated biphenyls (PCBs) represent a large group of recalcitrant environmental pollutants. Up to now, many studies have focused on bioremediation of PCBs by fungal strains; however, the mechanisms of adaptation of these strains towards PCBs remain unknown despite their importance in developing effective bioremediation processes. We studied five species, each consisting of two strains isolated either from PCB-polluted or PCB-unpolluted substrates (control strains). We investigated their responses to PCB contamination by studying their tolerance to PCBs, their ability to reduce these pollutants, and their expression level of Laccase genes. In Thermothelomyces thermophila, Thermothelomyces heterothallica, Thermoascus crustaceus, and Fusarium solani, all the studied strains showed a similar tolerance and PCB degradation regardless of their origin. In Schizophyllum commune, while both strains showed similar resistance to PCBs, i.e., PCBs and their degradation products presented no toxicity for these strains, the rate of PCB degradation of the strain from a PCB-polluted environment was significantly slightly higher. The PCB degradation did not correlate with the expression level of genes encoding Laccases. These results demonstrate that the tolerance and PCB degradation by the fungal strains, which did not involve Laccase genes, required different adaptation systems which seem to be constitutive or rapidly inducible by PCB according to the fungal species.

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Acknowledgments

We are deeply grateful to Viviane Barbreau for her critical reading of the manuscript and interesting remarks. We address our special thanks to Nael Mouhamadou, Ilian Mouhamadou, and Lila Mouhamadou for their help and unconditional support.

Funding

This research was financed by UMR 5553 CNRS/UGA “Projet interne”.

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Authors and Affiliations

  1. Laboratoire d’Ecologie Alpine, UMR 5553 CNRS/USMB Université Grenoble Alpes, 38058, Grenoble CEDEX 9, France

    Sophie Périgon, Martin Massier, Joaquim Germain, Marie-Noëlle Binet & Bello Mouhamadou

  2. Ecole de la Nature et du Paysage, INSA Centre Val de Loire, 9 Rue Chocolaterie, 41000, Blois, France

    Nicolas Legay

  3. CNRS, CITERES, UMR 7324, 37200, Tours, France

    Nicolas Legay

Authors
  1. Sophie Périgon
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  2. Martin Massier
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  3. Joaquim Germain
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  4. Marie-Noëlle Binet
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  5. Nicolas Legay
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  6. Bello Mouhamadou
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Corresponding author

Correspondence to Bello Mouhamadou.

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Responsible editor: Philippe Garrigues

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Périgon, S., Massier, M., Germain, J. et al. Metabolic adaptation of fungal strains in response to contamination by polychlorinated biphenyls. Environ Sci Pollut Res 26, 14943–14950 (2019). https://doi.org/10.1007/s11356-019-04701-5

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  • DOI: https://doi.org/10.1007/s11356-019-04701-5

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