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Efficiency of thermostable purified laccase isolated from Physisporinus vitreus for azo dyes decolorization

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Abstract

Laccases are versatile biocatalysts that are prominent for industrial purposes due to their extensive substrate specificity. Therefore, this research investigated producing laccase from Physisporinus vitreus via liquid fermentation. The results revealed that veratryl alcohol (4mM) was the most effective inducer 7500U/L. On the other hand, Zn ions inhibited laccase production. The optimum carbon and nitrogen sources were glucose and tryptone by 5200 and 3300 U/L, respectively. Moreover, solvents exhibited various impacts on the enzyme activity at three different solvent concentrations (5%, 10% and 20%), however, it showed a highest activity at 5% of the investigated solvent. Ferric ions inhibited the enzyme activity. In addition, the enzyme has a high ability to decolorize azo dyes when using syringaldehyde as a mediator. The purified laccase from Physisporinus vitreus is a promising substance to be used for industrial and environmental applications due to its stability under harsh conditions and efficiency in decolorization of dyes.

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Acknowledgements

The author thanks the Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

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Authors and Affiliations

  1. Department of Biology, College of Science, Taibah University, Madinah, 30001, Saudi Arabia

    Ahmad Alhujaily, Asmaa M. M. Mawad & Hibah M. Albasri

  2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Ahmad Alhujaily & Ma Fuying

  3. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt

    Asmaa M. M. Mawad

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AA performed the methodology, wrote the first draft and drew the figures and tables. AMMM edited and finalized the last version of the manuscript, HMA performed data analysis and curation. MF did supervision and conceptualization. All authors reviewed the final version of the manuscript.

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Alhujaily, A., Mawad, A.M.M., Albasri, H.M. et al. Efficiency of thermostable purified laccase isolated from Physisporinus vitreus for azo dyes decolorization. World J Microbiol Biotechnol 40, 138 (2024). https://doi.org/10.1007/s11274-024-03953-9

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