Abstract
Bacteria have the potential to adhere to abiotic surfaces, which has an undesirable effect in the food industry because they can survive for sustained periods through biofilm formation. In this study, an antibacterial peptide (ABP), with a molecular mass of 3861 Da, was purified from hydrolyzed chicken feathers using a locally isolated keratinolytic bacterium, namely Rhodococcus erythropolis, and its antibacterial and antibiofilm potential were investigated against planktonic and biofilm cells of Methicillin-Resistant Staphylococcus Aureus (MRSA). The results demonstrated that purified ABP showed the growth inhibition of MRSA cells with the minimum inhibitory concentration (MIC) of 45 µg/ml and disrupted MRSA biofilm formation at a concentration of 200 ug/ml, which results were confirmed by scanning electron micrograph (SEM). Moreover, the secondary structures of the peptide were assessed as part of the FTIR analysis to evaluate its mode of action. ExPASy tools were used to predict the ABP sequence, EPCVQUQDSRVVIQPSPVVVVTLPGPILSSFPQNTA, from a chicken feather keratin sequence database following in silico digestion by trypsin. Also, ABP had 54.29% hydrophobic amino acids, potentially contributing to its antimicrobial activity. The findings of toxicity prediction of the peptide by the ToxinPred tool revealed that ABP had non-toxic effects. Thus, these results support the potential of this peptide to be used as an antimicrobial agent for the treatment or prevention of MRSA biofilm formation in feed, food, or pharmaceutical applications.
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The datasets generated or analyzed during the current study are available from the corresponding author on reasonable request.
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The authors sincerely acknowledge the technical facilities provided by the University of Science and Technology-Faculty of Physics, Iran; Prof. Ulla’s lab at the Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, and the National Institute of Genetic Engineering and Biotechnology (NIGE), Iran. Also, the authors gratefully acknowledge Mr. Mojtaba Alahyaribeik for their financial support and forever encouragement toward research accomplishments.
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SA: conceived the study. SA and MN: performed experiments and analyzed data. SA: drafted all figures and did the statistical analysis. SA. and MN: wrote the manuscript. All authors approved the final manuscript.
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Alahyaribeik, S., Nazarpour, M. Peptide recovery from chicken feather keratin and their anti-biofilm properties against methicillin-resistant Staphylococcus aureus (MRSA). World J Microbiol Biotechnol 40, 123 (2024). https://doi.org/10.1007/s11274-024-03921-3
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DOI: https://doi.org/10.1007/s11274-024-03921-3