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Novel approach for the ammonium removal by simultaneous heterotrophic nitrification and denitrification using a novel bacterial species co-culture

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Abstract

Agricultural activities lead excessive emission of ammonia nitrogen in the environment and can profoundly interfere the equilibrium of the natural ecosystems leading to their contamination. Actually, the biological purification of wastewaters is the most adopted technique thanks to its several advantages such as high performance and low energy consumption. For this reason, two novel strains of Alcaligenes sp. S84S3 and Proteus sp. S19 genus were isolated from an activated sludge and applied in the treatment of ammonium and nitrite in aqueous solution. Under the optimum operating conditions of temperature (30 °C), pH (7), carbon substrate (2 g/L of glucose) and duration of incubation time (69 h), the strain Alcaligenes sp. S84S3 could oxidize 65 % of the ammonium as high as 272.72 mg-NH4 +/L. Moreover, during 48 h, the nitrate reduction rate performed by the strain Proteus S19 was about 99 % without production of nitrite intermediate (negligible concentration). Moreover, the coculture of the strains Alcaligenes sp. S84S3 and Proteus sp. S19 could eliminate 65.83 % of the ammonium ions without production of toxic forms of nitrogen oxides during a short time of incubation (118 h) at the same operational conditions with providing the aeration in the first treatment phase. The coculture of our isolated strains is assumed to have a good potential for nitrification and denitrification reactions applied in the treatment of wastewater containing ammonium, nitrite and nitrate. As a result, we can consider that the mixed culture is a practical method in the treatment of high-strength ammonium wastewater with reducing of sludge production.

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Acknowledgments

The authors thank Prof. Amina Khemili and Assia Benakouche for their careful reading.

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Authors and Affiliations

  1. Laboratory of Treatment and Formatting of Polymers, Faculty of Engineering Sciences, University of M’Hamed Bougara, 35000, Boumerdes, Algeria

    Yassmina Angar & Nacer-Eddine Djelali

  2. Department of Biology, Laboratory of VALCOR, Faculty of Sciences, University of M’Hamed Bougara, 35000, Boumerdes, Algeria

    Salima Kebbouche-Gana & Souad Khemili-Talbi

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  1. Yassmina Angar
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  2. Salima Kebbouche-Gana
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  3. Nacer-Eddine Djelali
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  4. Souad Khemili-Talbi
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Correspondence to Yassmina Angar.

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Angar, Y., Kebbouche-Gana, S., Djelali, NE. et al. Novel approach for the ammonium removal by simultaneous heterotrophic nitrification and denitrification using a novel bacterial species co-culture. World J Microbiol Biotechnol 32, 36 (2016). https://doi.org/10.1007/s11274-015-2007-y

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