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A new xylanase from thermoalkaline Anoxybacillus sp. E2 with high activity and stability over a broad pH range

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Abstract

A xylanase gene, xynE2, was cloned from thermoalkaline Anoxybacillus sp. E2 and was expressed in Escherichia coli BL21 (DE3). The gene consisted of 987 bp and encoded a 328-residue xylanase with a calculated molecular weight of 38.8 kDa. On the basis of amino acid sequence similarities, this enzyme was assigned as a member of glycoside hydrolase family 10. Purified recombinant XynE2 showed maximal activity at pH 7.8 and 65°C, and was thermostable at 60°C. The enzyme was highly active and stable over a broad pH range, showing more than 90% of maximal activity at pH 6.6–pH 8.6 and retaining more than 80% of activity at pH 4.6–pH 12.0, 37°C for 1 h, respectively. These favorable properties make XynE2 a good candidate in the pulp and paper industries. This is the first report on gene cloning, expression and characterization of a xylanase from the genus Anoxybacillus.

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Acknowledgment

This work was supported by the National High Technology Research and Development Program of China (863 Program; grant no. 2007AA100601) and the National Key Technology Program of China (grant no. 2006BAD12B05-03).

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Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China

    Jianshe Wang, Yingguo Bai, Peilong Yang, Pengjun Shi, Huiying Luo, Kun Meng, Huoqing Huang & Bin Yao

  2. College of Bioengineering, Inner Mongolia Agricultural University, 010018, Hohhot, People’s Republic of China

    Jianshe Wang & Jun Yin

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  1. Jianshe Wang
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  2. Yingguo Bai
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  9. Bin Yao
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Correspondence to Bin Yao.

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Wang, J., Bai, Y., Yang, P. et al. A new xylanase from thermoalkaline Anoxybacillus sp. E2 with high activity and stability over a broad pH range. World J Microbiol Biotechnol 26, 917–924 (2010). https://doi.org/10.1007/s11274-009-0254-5

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  • DOI: https://doi.org/10.1007/s11274-009-0254-5

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