Abstract
The aim of this study was to evaluate the effects of different selenium compounds on the sperm quality of cryopreserved ram semen. Ejaculates from four rams, collected using an artificial vagina heated to 38 °C, were individually evaluated. The approved ejaculates were pooled and diluted (1:1 v:v) in Tris-egg yolk extender (20%, v/v) and separated into two control groups, one cooled for 2 h and the other for 4 h. The pooled ejaculates at the two cooling periods were supplemented with two doses (0.5 and 1 μg/mL) of organic selenium (ORG), and inorganic selenium (SeNa), each. The samples were packed in 0.25 ml straws, at a concentration of 400 × 106 sperms/mL and stored in liquid nitrogen. The straws were thawed in a water bath at 37 °C for 20 s, and the samples were subjected to sperm kinetics evaluation by Computer Assisted Semen Analysis software. Sperm membrane integrity, acrosome morphology, and mitochondrial potential were assessed. In addition, oxidative stress markers reactive oxygen species (ROS), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive species (TBARS), and glutathione peroxidase (GPx) enzyme activity) were also evaluated. No significant improvement was observed in the ram semen quality at the two cooling times. Supplementation of the freezing extender with 0.5 μg/mL ORG, subjected to 4 h cooling period, increased the sperm motility when compared with the control group at the same cooling time. In addition, the 0.5 μg/mL SeNa group, under the 2 h cooling period, showed an increase in sperm motility when compared to the control group at the same cooling period. Considering the importance of sperm motility as a fertility parameter, our study indicates that supplementation with ORG and SeNa can help improve the total motility of the cryopreserved ram semen.
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The datasets generated during and/or analysed during the current study will be made available on reasonable request.
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Acknowledgements
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Brasil– Finance Code 001. The National Council for Scientific and Technological Development (CNPq) is acknowledged for financial support to F.W.S.C., and CAPES and FAPERGS are acknowledged for financial support to J.F.R. (M.Sc. Fellowships) and to J.B.R. (Dr. Fellowships).
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Conceived and designed the experiments: J.F.R., D.S.B., L.C., D.S.L., F.W.S.C.; performed the experiments: J.F.R., L.C., D.F.B., F.W.S.C.; performed semen analysis: J.F.R., L.C., D.S.B.; performed the selenofuranoside synthesis: D.S.L.; analyzed the data: J.F.R., L.C., F.W.S.C., D.S.B.; wrote the manuscript: J.F.R., F.W.S.C. All the authors have approved the final manuscript.
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This study was approved by the ethics committee of the Federal University of Pampa (017/2019) for the use of animals.
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Rodrigues, J.F., Dacampo, L.D.L., Bicca, D.F. et al. Effects of organic and inorganic selenium supplementation on frozen-thawed ram semen at two cooling periods. Vet Res Commun (2024). https://doi.org/10.1007/s11259-024-10302-6
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DOI: https://doi.org/10.1007/s11259-024-10302-6