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Molecular characterization and phylogenetic analysis of infectious bursal disease viruses isolated from chicken in South China in 2011

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Abstract

Infectious bursal disease virus (IBDV) is a double-stranded RNA virus that causes immunosuppressive disease in young chickens. Thousands of cases of IBDV infection are reported each year in South China, and these infections can result in considerable economic losses to the poultry industry. To monitor variations of the virus during the outbreaks, 30 IBDVs were identified from vaccinated chicken flocks from nine provinces in South China in 2011. VP2 fragments from different virus strains were sequenced and analyzed by comparison with the published sequences of IBDV strains from China and around the world. Phylogenetic analysis of hypervariable regions of the VP2 (vVP2) gene showed that 29 of the isolates were very virulent (vv) IBDVs, and were closely related to vvIBDV strains from Europe and Asia. Alignment analysis of the deduced amino acid (aa) sequences of vVP2 showed the 29 vv isolates had high uniformity, indicated low variability and slow evolution of the virus. The non-vvIBDV isolate JX2-11 was associated with higher than expected mortality, and had high deduced aa sequence similarity (99.2 %) with the attenuated vaccine strain B87 (BJ). The present study has demonstrated the continued circulation of IBDV strains in South China, and emphasizes the importance of reinforcing IBDV surveillance.

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Acknowledgments

This work was supported by a grant (no. 2009B020201008) from Guangdong Momentously Scientific and Technological Project, and a grant (no. 2010B090301019) from Strategic Cooperation Project of Guangdong Province and Chinese Academy.

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Authors and Affiliations

  1. College of Animal Science, South China Agricultural University, Guangzhou, 510642, China

    Di Liu, Xiang-Bin Zhang, Zhuan-Qiang Yan, Feng Chen, Jun Ji, Yu Xue, Jia-Jia Liu, Qing-Mei Xie, Jing-Yun Ma & Ying-Zuo Bee

  2. Guangdong Wen’s Foodstuffs Group Co. Ltd, Yunfu, 527439, China

    Di Liu, Jian-Ping Qin, Hai-Yan Li & Jun-Peng Lu

  3. State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-Sen University, Guangzhou, 510006, China

    Chun-Yi Xue

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  1. Di Liu
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  2. Xiang-Bin Zhang
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  5. Jun Ji
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  13. Chun-Yi Xue
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  14. Ying-Zuo Bee
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Corresponding author

Correspondence to Feng Chen.

Additional information

Di Liu and Xiang-Bin Zhang contributed equally to this study.

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Liu, D., Zhang, XB., Yan, ZQ. et al. Molecular characterization and phylogenetic analysis of infectious bursal disease viruses isolated from chicken in South China in 2011. Trop Anim Health Prod 45, 1107–1112 (2013). https://doi.org/10.1007/s11250-012-0333-8

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  • DOI: https://doi.org/10.1007/s11250-012-0333-8

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