Skip to main content
SpringerLink
Log in

Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D3, E, and H supplementation

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

The effect of vitamins A, D3, E, and H supplementation on oxidative stress indices in Indian water buffaloes suffering from subclinical mastitis was investigated. Changes in the total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), and activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) in milk were evaluated before and after the supplementation of vitamins A, D3, E, and H. The buffaloes suffering from subclinical mastitis revealed remarkable alterations in the milk oxidants/antioxidants balance shifted towards oxidative status. The buffaloes with subclinical mastitis revealed significantly (P ≤ 0.01) higher TOC, NO contents, and CAT activity, while TAC content and GSH-Px activity were significantly (P ≤ 0.01) lower in comparison with the healthy controls. However, SOD activity did not show any significant change. Supplementation of vitamins A, D3, E, and H to these animals revealed significant (P ≤ 0.01) reduction in TOC, NO, and CAT, while a significant (P ≤ 0.01) increase in TAC and GSH-Px activity was also evident. From the present study, it may be concluded that supplementation of these vitamins can help ameliorate the altered milk oxidants/antioxidants balance towards normalcy and, thus, ensue recovery from subclinical mastitis in the Indian water buffaloes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Aarestrup, F.M., 2005. Veterinary drug usage and antimicrobial resistance in bacteria of animal origin, Basic and Clinical Pharmacology and Toxicology, 96, 271–281.

    Article  PubMed  CAS  Google Scholar 

  • Andrei, S., Matei, S., Fit, N., Cernea, C., Ciupe, S., Bogdan, S. and Groza, I.S., 2011. Glutathione peroxidase activity and its relationship with somatic cell count, number of colony forming units and protein content in subclinical mastitis cows milk, Romanian Biotechnological Letters, 16 (3), 6209–6217.

    CAS  Google Scholar 

  • Antunes, L.M.G., Pascoala, L.M., Bianchib, M.L.P. and Diasa, F.L., 2005. Evaluation of the clastogenicity and anticlastogenicity of the carotenoid bixin in human lymphocyte cultures, Mutation Research Genetic Toxicology and Environtal Mutagen, 585(1–2), 113–119.

    Article  CAS  Google Scholar 

  • Atakisi, O., Oral, H., Atakisi, E., Mehran, O., Pancarci, S. M., Ozcan, A., Marasil, S., Polat, B., Colak, A. and Kaya, S., 2010. Subclinical mastitis causes alternations in nitric oxide, total oxidant and antioxidant capacity in cow milk, Research in Veterinary Scieience, 89, 10–13.

    Article  CAS  Google Scholar 

  • Barbano, D.M., Ma, Y. and Santos, M.V., 2006. Influence of raw milk quality on fluid milk shelf life, Journal of Dairy Science, 89, E15–E19.

    Article  PubMed  Google Scholar 

  • Boulanger, V., Zhao, X. and Lacasse, P., 2002. Protective effect of melatonin and catalase in bovine neutrophil-induced model of mammary cell damage, Journal of Dairy Science, 85, 562–569.

    Article  PubMed  CAS  Google Scholar 

  • Bourne, N., Wathes, D.C., Lawrence, K.E., McGowan, M. and Laven R.A., 2008. The effect of parenteral supplementation of vitamin E with selenium on the health and productivity of dairy cattle in the UK, The Veterinary Journal, 177, 381–387.

    Article  PubMed  CAS  Google Scholar 

  • Celi, P., 2011. Biomarkers of oxidative stress in ruminant medicine (Review), Immunopharmacology and Immunotoxicology, 33(2), 233–240.

    Article  PubMed  Google Scholar 

  • Cemek, M., Caksen, H., Bayiroglu, F., Cemek, F. and Dede, S., 2006. Oxidative stress and enzymic-non-enzymic antioxidant responses in children with acute pneumonia, Cell Biochemistry and Function, 24(3), 269–273.

    Article  PubMed  CAS  Google Scholar 

  • Choi, J., 2012. Oxidative stress, endogenous antioxidants, alcohol, and hepatitis C: pathogenic interactions and therapeutic considerations, Free Radical Biology & Medicine, 52, 1135–1150.

    Article  CAS  Google Scholar 

  • Cruz, L.C., 2010. Recent developments in the buffalo industry of Asia. Proceeding of the 9th World Buffalo Congress, April 25–28, 2010, Argentina, pp: 7–19.

  • DeVliegher, S., Fox, L.K., Piepers, S., McDougall, S. and Barkema, H.W., 2012. Invited review: Mastitis in dairy heifers: Nature of the disease, potential impact, prevention, and control (Review), Journal of Dairy Science, 95(3), 1025–1040.

    Article  CAS  Google Scholar 

  • DeLuca, H., 2004. Overview of general physiologic features and functions of vitamin D, The American Journal of Clinical Nutrition, 80(Suppl), 1689S–1696S.

    PubMed  CAS  Google Scholar 

  • Deluyker, H.A., Van Oye, S.N. and Boucher, J.F., 2005. Factors affecting cure and somatic cell count after pirlimycin treatment of subclinical mastitis in lactating cows, Journal of Dairy Science, 88, 604–614.

    Article  PubMed  CAS  Google Scholar 

  • Dimri, U., Sharma, M.C., Yamdagni, A., Ranjan, R. and Zama, M.M.S., 2010. Psoroptic mange infestation increases oxidative stress and decreases antioxidant status in sheep, Veterinary Parasitology, 168 (3–4), 318–322.

    Article  PubMed  CAS  Google Scholar 

  • Erel, O., 2004. A novel automated method to measure total antioxidant response against potent free radical reactions, Clinical Biochemistry, 37, 112–119.

    Article  PubMed  CAS  Google Scholar 

  • Erel, O., 2005. A new automated colorimetric method for measuring total oxidant status, Clinical Biochemistry, 38, 1103–1111.

    Article  PubMed  CAS  Google Scholar 

  • Hicks, C.L., 1980. Occurrence and consequence of superoxide dismutase in milk products: a review, Journal of Dairy Science, 63, 1199–1204.

    Article  PubMed  CAS  Google Scholar 

  • Kanbur, M., Atalay, O., Lea, A., Eraslan, G. and Cam, Y., 2008. The curative and antioxidative efficiency of doramectin and doramectin plus vitamin AD3E treatment on Psoroptes cuniculi infestation in rabbits, Research in Veterinary Science, 85, 291–293.

    Article  PubMed  CAS  Google Scholar 

  • Kankofer, M., Albera, E., Feldman, M., Gundling, N. and Hoedemaker, M., 2010. Comparison of antioxidative/oxidative profiles in blood plasma of cows with and without retained fetal placental membranes, Theriogenology, 74, 1385–1395.

    Article  PubMed  CAS  Google Scholar 

  • Kara, H., Cevik, A., Konar, V., Dayangac, A. and Servi, K., 2008. Effects of selenium with vitamin E and melatonin on cadmium-induced oxidative damage in rat liver and kidneys, Biological Trace Element Research, 125, 236–244.

    Article  PubMed  CAS  Google Scholar 

  • Kasravi, R., Bolourchi, M., Farzaneh, N., Seifi, A.H., Barin, A., Hovareshti, P. and Gharagozlou, F., 2012. Efficacy of conventional and extended intra-mammary treatment of persistent sub-clinical mastitis with cefquinome in lactating dairy cows, Tropical Animal Health and Production, 43, 1203–1210.

    Article  Google Scholar 

  • Leterrier, M., Airaki, M., Palma, J.M., Chaki, M., Barroso, J.B. and Corpas, F.J., 2012. Arsenic triggers the nitric oxide (NO) and S-nitrosoglutathione (GSNO) metabolism in Arabidopsis, Environmental Pollution, 166, 136–143.

    Article  PubMed  CAS  Google Scholar 

  • Lipko-Przybylska, J. and Kankofer, M., 2012. Antioxidant defence of colostrum and milk in consecutive lactations in sows, Irish Veterinary Journal, 65 (4), 2–8.

    Google Scholar 

  • Lykkesfeldt J. and Svendsen O., 2007. Oxidants and antioxidants in disease: Oxidative stress in farm animals, The Veterinary Journal, 173, 502–511.

    Article  PubMed  CAS  Google Scholar 

  • McDougall, S., Parker, K.I., Heuer, C. and Compton, C.W.R., 2009. A review of prevention and control of heifer mastitis via non-antibiotic strategies, Veterinary Microbiology, 134, 177–185.

    Article  PubMed  CAS  Google Scholar 

  • Miranda, K.M., Espey, M.G. and Wink, D.A., 2001. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite, Nitric Oxide, 5, 62–71.

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee, R., 2008. Selenium and vitamin E increases polymorphonuclear cell phagocytosis and antioxidant levels during acute mastitis in riverine buffaloes, Veterinary Research Communications, 32, 305–313.

    Article  PubMed  Google Scholar 

  • Mweu M.M., Nielsen, S.S., Halasa T. and Toft N., 2012. Annual incidence, prevalence and transmission characteristics of Streptococcus agalactiae in Danish dairy herds, Preventive Veterinary Medicine, 106, 244–250. http://dx.doi.org/10.1016/j.prevetmed.2012.04.002

  • O’Rourke, D., 2009. Nutrition and udder health in dairy cows: a review, Irish Veterinary Journal, 62, 15–20.

    Article  Google Scholar 

  • Pitkala, A., Haveri, M., Pyorala, S., Myllys, V. and Honkanen-Buzalski, T., 2004. Bovine mastitis in Finland 2001—Prevalence, distribution of bacteria, and antimicrobial resistance, Journal of Dairy Science, 87, 2433–2441.

    Article  PubMed  CAS  Google Scholar 

  • Ranjan, R., Swarup, D., Naresh, R. and Patra, R.C., 2005. Enhanced erythrocytic lipid peroxides and reduced plasma ascorbic acid, and alteration in blood trace elements level in dairy cows with mastitis, Veterinary Research Communications, 29, 27–34.

    Article  PubMed  CAS  Google Scholar 

  • Rizzo, A., Ceci, E., Pantaleo, M., Mutinati, M., Spedicato, M., Minoia, G. and Sciorsci, R L., 2012. Evaluation of blood and milk oxidative status during early postpartum of dairy cows, Animal, 8, 1–6.

    Google Scholar 

  • Said, H.M., 2012. Biotin: biochemical, physiological and clinical aspects. Subcellular Biochemistry, 56,1–19.

    Article  PubMed  CAS  Google Scholar 

  • Salman, S., Khol-Parisini, A., Schafft, H., Lahrssen-Wiederholt, M., Hulan, H. W., Dinse, D., Zentek, J., 2009. The role of dietary selenium in bovine mammary gland health and immune function, Animal Health Research Review, 10, 21–34.

    Article  CAS  Google Scholar 

  • Schukken Y. H., Gunther, J., Fitzpatrick, J., Fontaine, M.C., Goetze, L., Holst, O., Leigh, J., Petzl, W., Schuberth, H.J., Sipka, A., Smith, D.G.E., Quesnell, R., Watts, J., Yancey, R., Zerbe, H., Gurjar, A., Zadoks, R.N. and Seyfert, H.M., 2012. Host-response patterns of intramammary infections in dairy cows, Veterinary Immunology and Immunopathology, 144, 270–289.

    Article  Google Scholar 

  • Sharma, B. and Sharma, S.D., 2009. Antioxidants: tools to enhance livestock productivity, The Indian Cow, (Jan.–March), 3–15.

  • Sharma, N., Rho, G.J., Hong, Y.H., Kang, T.Y., Lee, H.K., Hur, T. and Jeong, D.K., 2012. Bovine Mastitis: An Asian Perspective, Asian Journal of Animal and Veterinary Advances, 7, 454–476.

    Article  Google Scholar 

  • Silanikove, N., Rauch-Cohen, A., Shapiro, F., Arieli, A., Merin, U. and Leitner, G., 2012. Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability, Animal, 6, 1451–1459.

    Article  PubMed  CAS  Google Scholar 

  • Singh, S.K., Dimri, U., Sharma, M.C., Swarup, D., Kumar, M. and Tiwari, R., 2012. Psoroptes cuniculi induced oxidative imbalance in rabbits and its alleviation by using vitamins A, D3, E, and H as adjunctive remedial, Tropical Animal Health and Production, 44, 43–48.

    Article  PubMed  Google Scholar 

  • Sordillo, L.M. and Aitken, S.L., 2008. Impact of oxidative stress on the health and immune function of dairy cattle, Veterinary Immunology and Immunopathology, 128(1–3), 104–109.

    PubMed  Google Scholar 

  • Sordillo, L.M., 2009. Current Concepts on Immunity and Mastitis, WCDS Advanced Dairy Technology, 21, 111–119.

    Google Scholar 

  • Spears, J.W. and Weiss, W.P., 2008. Role of antioxidants and trace elements in health and immunity of transition dairy cows, The Veterinary Journal, 176, 70–76.

    Article  PubMed  CAS  Google Scholar 

  • Tesfaye, G.Y., Regassa, F.G. and Kelay, B., 2010. Milk yield and associated economic losses in quarters with subclinical mastitis due to Staphylococcus aureus in Ethiopian crossbred dairy cows, Tropical Animal Health and Production, 42, 925–931.

    Article  PubMed  Google Scholar 

  • Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T.D., Mazur, M. and Telser, J., 2007. Free radicals and antioxidants in normal physiological functions and human disease, International Journal of Biochemistry & Cell Biology, 39, 44–84.

    Article  CAS  Google Scholar 

  • Zhao, X., Lacasse, P., 2008. Mammary tissue damage during bovine mastitis: Causes and control, Journal of Animal Science, 86, 57–65.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Shanker K. Singh

    Present address: Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, 281001, UP, India

Authors and Affiliations

  1. Division of Medicine, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, UP, India

    Umesh Dimri, Mahesh Chandra Sharma, Shanker K. Singh, Pankaj Kumar, Ricky Jhambh, Bishwambhar Singh, Samiran Bandhyopadhyay & Med Ram Verma

  2. Livestock Economics and Statistics, Indian Veterinary Research Institute, Bareilly, India

    Bishwambhar Singh & Med Ram Verma

  3. Eastern Regional Station, Kolkata, India

    Samiran Bandhyopadhyay

Authors
  1. Umesh Dimri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mahesh Chandra Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shanker K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pankaj Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ricky Jhambh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bishwambhar Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Samiran Bandhyopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Med Ram Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Umesh Dimri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dimri, U., Sharma, M.C., Singh, S.K. et al. Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D3, E, and H supplementation. Trop Anim Health Prod 45, 971–978 (2013). https://doi.org/10.1007/s11250-012-0319-6

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-012-0319-6

Keywords

Search

Navigation