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Effects of methyl jasmonate and abscisic acid on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana)

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Abstract

We researched the effects of methyl jasmonate, abscisic acid, and a combination of the two on anthocyanin metabolism in callus material induced from the leaves of a red-fleshed apple individual, which was a hybrid offspring of Malus sieversii f. niedzwetzkyana and Malus domestica cv. ‘Fuji’. The results showed that methyl jasmonate promoted anthocyanin accumulation with increasing methyl jasmonate concentration in the range of 10−6 to 10−3 mol/L. Abscisic acid significantly inhibited anthocyanin biosynthesis, and its inhibitory effect was highest at 6 μmol/L among all treatments. In addition, methyl jasmonate alleviated the inhibitory effect of abscisic acid on anthocyanin biosynthesis. Gene expression analysis indicated that methyl jasmonate induced the expression of MdMYB9 and MdMYB10, which are positive regulator of anthocyanin biosynthesis, and inhibited MdMYB11 expression. It also upregulated the expression of the anthocyanin structural genes MdCHS, MdF3H and MdUFGT. Abscisic acid inhibited the expression of MdMYB3 and MdMYB10, as well as the expression of the structural genes MdF3H, MdDFR, MdLDOX and MdUFGT. Methyl jasmonate relieved the inhibition by abscisic acid by decreasing the expression of MdMYB11 and MdMYB16, and increasing the expression of the anthocyanin regulatory genes MdMYB3, MdMYB9 and MdMYB10, and the structural genes MdCHS, MdDFR, MdF3H and MdUFGT. Thus, we elucidated the roles of methyl jasmonate and abscisic acid in the regulation of anthocyanin production at the gene transcription level.

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Acknowledgements

The work was supported by grants from the National Natural Science Foundation of China (CN) (31572091), the National key research and development project (2016YFC0501505), the National Natural Science Foundation of China (31201593), and the Natural Science Foundation of Shandong Province, China (ZR2015CQ016).

Author contributions

Conceived and designed the experiments: JJS, YCW, SQF, YLW. Performed the experiments: JJS. Analyzed the data: JJS, YCW. Contributed to the writing of the manuscript: JJS, YCW, SQF, YLW.

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Authors and Affiliations

  1. College of Forestry, Shandong Agricultural University, Tai-An, Shandong, China

    Jingjing Sun, Xiaojun Gong, Li Ma, Yanfen Qiu & Yanling Wang

  2. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China

    Yicheng Wang, Xuesen Chen, Nan Wang & Shouqian Feng

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  1. Jingjing Sun
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  2. Yicheng Wang
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Correspondence to Yanling Wang or Shouqian Feng.

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Jingjing Sun and Yicheng Wang: Co-first author.

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Supplementary Table S1. Primer sequences used for the qRT-PCR validation (DOCX 14 KB)

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Sun, J., Wang, Y., Chen, X. et al. Effects of methyl jasmonate and abscisic acid on anthocyanin biosynthesis in callus cultures of red-fleshed apple (Malus sieversii f. niedzwetzkyana). Plant Cell Tiss Organ Cult 130, 227–237 (2017). https://doi.org/10.1007/s11240-017-1217-4

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