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Genetic transformation and molecular research in Anthurium: progress and prospects

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Abstract

Although Anthurium is an attractive and commercially popular ornamental plant, its genetic enhancement has lagged behind that of other ornamental crops. There are several agronomically important traits in need of improvement. These include novel flower colors and morphologies, increased shelf and vase lives, and resistance to bacterial blight (Xanthomonas axonopodis pv. dieffenbachiae), burrowing nematodes and abiotic stresses. The production of transgenic Anthuriums is critical because the conventional breeding of a cultivar with beneficial traits typically requires 8–10 years. This review evaluates the problems, challenges and progress associated with developing molecular markers for Anthurium and in genetically transforming this ornamental. Recent improvements have hastened the tissue culture and regeneration of transgenic plants primarily using Agrobacterium-based methods. Promoter analyses have focused on constitutive and tissue-enhanced gene expression with the green fluorescent protein being a more reliable reporter than β-glucuronidase. The development of molecular markers assists with phylogenetic analyses and PCR-based markers such as RAPD, SSR, SPAR, ISSR and AFLP can be used to differentiate cultivars and for genetic fingerprinting. The marker-assisted breeding of Anthurium will become more feasible once available data are used for association to specific traits. Work on the identification of quantitative trait loci for disease resistance and other traits such as flower colour is required and should incorporate new approaches, such as next-generation sequencing technologies. By highlighting the aforementioned bottlenecks and successes in this review, it is expected that the pace of Anthurium genetic improvement will increase with the multifaceted incorporation of focused priorities and new technology advancements.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

ANS:

Anthocyanidin synthase

AS:

Acetosyringone

bHLH:

Basic helix-loop-helix family

CaMV35S:

Cauliflower mosaic virus 35S promoter

CHI:

Chalcone isomerase

CHS:

Chalcone synthase

DFR:

Dihydroflavonol 4-reductase

EST:

Expressed sequence tag

F3H:

Flavanone 3-hydroxylase

F3′H:

Flavonoid 3′-hydroxylase

GFP:

Green fluorescent protein

GUS:

β-Glucuronidase

ISSR:

Inter simple sequence repeat

MAS:

Marker-assisted breeding

nptII :

Neomycin phosphotransferase II

PCR:

Polymerase chain reaction

RAPD:

Random amplified polymorphic DNA

SPAR:

Single primer amplification reaction

Xad :

Xanthomonas axonopodis pv. dieffenbachiae

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Authors and Affiliations

  1. P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa-Ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Research Institute of Nyíregyháza, University of Debrecen, Nyíregyháza, P.O. Box 12, 4400, Hungary

    Judit Dobránszki

  3. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Songjun Zeng

  4. Tissue Culture Department, Indonesian Ornamental Crops Research Institute, Jl. Raya Ciherang, Pacet-Cianjur, West Java, 43253, Indonesia

    Budi Winarto

  5. Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine Campus, College Road, St. Augustine, Republic of Trinidad and Tobago

    Adrian M. Lennon

  6. Biotechnology Department, Faculty of Agriculture, University of Mauritius, Reduit, Mauritius

    Yasmina Jaufeerally-Fakim

  7. Department of Molecular Biosciences & Bioengineering, University of Hawaii-Manoa, 1955 East-West Road, Ag. Science 218, Honolulu, HI, 96822, USA

    David A. Christopher

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Teixeira da Silva, J.A., Dobránszki, J., Zeng, S. et al. Genetic transformation and molecular research in Anthurium: progress and prospects. Plant Cell Tiss Organ Cult 123, 205–219 (2015). https://doi.org/10.1007/s11240-015-0832-1

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