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In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt

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Abstract

Fusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt.

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Acknowledgments

We are grateful to Dr. F. Laranjeira for providing the isolate of Fusarium oxysporum f. sp. passiflorae. This research was financed by the National Council for Scientific and Technological Development (CNPq, Brazil).

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Authors and Affiliations

  1. Centro de Pesquisa Agroflorestal Acre, Empresa Brasileira de Pesquisa Agropecuária, Embrapa, Rodovia BR-364, km 14, Caixa Postal, 32169908-970, Rio Branco, AC, Brazil

    Patrícia Silva Flores, Telma Miranda dos Santos & Claudio Horst Bruckner

  2. Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil

    Wagner Campos Otoni

  3. Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Brazil

    Onkar Dev Dhingra

  4. Centro de Ciências Biológicas, Departamento de Bioquímica, Universidade Estadual de Maringá, 87029-900, Maringá, PR, Brazil

    Sérgio Paulo Severo de Souza Diniz

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  1. Patrícia Silva Flores
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  2. Wagner Campos Otoni
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  3. Onkar Dev Dhingra
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  4. Sérgio Paulo Severo de Souza Diniz
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  5. Telma Miranda dos Santos
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  6. Claudio Horst Bruckner
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Correspondence to Patrícia Silva Flores.

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Flores, P.S., Otoni, W.C., Dhingra, O.D. et al. In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt. Plant Cell Tiss Organ Cult 108, 37–45 (2012). https://doi.org/10.1007/s11240-011-0009-5

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