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Genetic Diversity and Identity of Chinese Loquat Cultivars/Accessions (Eriobotrya japonica) Using Apple SSR Markers

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Abstract

Loquat (Eriobotrya japonica) is an underutilized fruit crop that originated in China and for which only a small number of molecular markers are available. This number can be increased by identifying apple SSRs that are transferable to loquat cultivars/accessions to provide new insight into the level of genetic diversity within loquat and synteny with apple. We evaluated 71 apple SSR markers distributed across 17 linkage groups, and identified 39 SSRs transferable to loquat. Testing 54 loquat accessions, from Japan, Spain, four provinces in China, and two wild species gave a total of 155 different alleles with a mean value of 3.38 per locus. The mean effective number of alleles was 2.21, and the mean observed heterozygosity was 0.47. These values indicate a high degree of genetic diversity in the set of Chinese loquat accessions analyzed. Unweighted pair-group method analysis based on simple matching coefficent clustered the accessions into two groups, cultivated and wild loquat. The cultivated loquat can be subdivided into three subgroups which generally reflect their geographic origin in China. The Spanish cultivars clustered with those of the Jiangsu and Zhejiang provinces. A core set of five SSR markers could distinguish most accessions.

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Acknowledgments

This work was supported by the Special Research Fund for Public Welfare in Chinese Agriculture (contract no. 200903041) and the Ministry of Education of the PR China Visiting PhD Students, also by Southwest University Postgraduate Science and Technology Innovation Foundation Projects (contract no. 2006013). We gratefully acknowledge Lin S.Q. of South China Agricultural University and Wu J.C. of Putian College for kindly providing the Spanish and Fujian Province accessions, respectively. We thank Dr. Luud Gilissen for his critical comments.

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Authors and Affiliations

  1. College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400716, China

    Qiao He, Guo Lu Liang, Kun Ji & Qi Gao Guo

  2. Department of Horticulture, Laboratory of Horticultural Plant Growth, Development and Quality Improvement, State Ministry of Agriculture, Zhejiang University, Hangzhou, 310029, China

    Qiao He, Xiong Wei Li, Kun Song Chen & Zhong Shan Gao

  3. Suzhou Polytechnic Institute of Agriculture, Suzhou, 215008, China

    Wei Min Yuan

  4. Loquat Institute, Yuhang District, Hangzhou, 311106, China

    Gen Zhi Zhou

  5. Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

    W. Eric van de Weg

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  1. Qiao He
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  2. Xiong Wei Li
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  3. Guo Lu Liang
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  7. Gen Zhi Zhou
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  8. Kun Song Chen
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  9. W. Eric van de Weg
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  10. Zhong Shan Gao
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Correspondence to Guo Lu Liang or Zhong Shan Gao.

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He, Q., Li, X.W., Liang, G.L. et al. Genetic Diversity and Identity of Chinese Loquat Cultivars/Accessions (Eriobotrya japonica) Using Apple SSR Markers. Plant Mol Biol Rep 29, 197–208 (2011). https://doi.org/10.1007/s11105-010-0218-9

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