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Herbaspirillum rubrisubalbicans, a mild pathogen impairs growth of rice by augmenting ethylene levels

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Abstract

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Herbaspirillum rubrisubalbicans decreases growth of rice. Inoculation of rice with H. rubrisubalbicans increased the ACCO mRNA levels and ethylene production. The H. rubrisubalbicans rice interactions were further characterized by proteomic approach.

Abstract

Herbaspirillum rubrisubalbicans is a well-known growth-promoting rhizobacteria that can also act as a mild phyto-pathogen. During colonisation of rice, RT-qPCR analyses showed that H. rubrisubalbicans up-regulates the methionine recycling pathway as well as phyto-siderophore synthesis genes. mRNA levels of ACC oxidase and ethylene levels also increased in rice roots but inoculation with H. rubrisubalbicans impaired growth of the rice plant. A proteomic approach was used to identify proteins specifically modulated by H. rubrisubalbicans in rice and amongst the differentially expressed proteins a V-ATPase and a 14-3-3 protein were down-regulated. Several proteins of H. rubrisubalbicans were identified, including the type VI secretion system effector Hcp1, suggesting that protein secretion play a role colonisation in rice. Finally, the alkyl hydroperoxide reductase, a primary scavenger of endogenous hydrogen peroxide was also identified. Monitoring the levels of reactive oxygen species in the epiphytic bacteria by flow cytometry revealed that H. rubrisubalbicans is subjected to oxidative stress, suggesting that the alkyl hydroperoxide reductase is an important regulator of redox homeostasis in plant-bacteria interactions.

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Acknowledgements

We thank Roseli Prado, Valter Baura and Marilza Doroti Lamour for technical assistance. We are indebted to William J. Broughton for critical reading of the manuscript and Geraldo Picheth for help with the statistical analysis. The National Institute of Science and Technology of Nitrogen Fixation/CNPq/MCT and Fundação Araucária provided financial support. G.V. thanks PNPD/CAPES for postdoctoral scholarship.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Centro Politécnico, Setor de Ciências Biológicas, Jardim das Américas, Federal University of Parana, Curitiba, 19046, PR, Brazil

    Glaucio Valdameri, Dayane Alberton, Vivian Rotuno Moure, Thiago Borba Kokot, Caroline Kukolj, Liziane Cristina Campos Brusamarello-Santos, Rose Adele Monteiro, Fabio de Oliveira Pedrosa & Emanuel Maltempi de Souza

  2. Department of Clinical Analysis, Federal University of Parana, Curitiba, PR, Brazil

    Glaucio Valdameri & Dayane Alberton

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  1. Glaucio Valdameri
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  2. Dayane Alberton
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  3. Vivian Rotuno Moure
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  4. Thiago Borba Kokot
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Contributions

GV data collection, data analysis and drafting the article. DA, VRM, TBK, CK, LCCBS data collection and analysis. RAM and FOP design of the work and critical revision of the article. EMS conception of the work, data interpretation and critical revision of the article.

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Correspondence to Emanuel Maltempi de Souza.

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Valdameri, G., Alberton, D., Moure, V.R. et al. Herbaspirillum rubrisubalbicans, a mild pathogen impairs growth of rice by augmenting ethylene levels. Plant Mol Biol 94, 625–640 (2017). https://doi.org/10.1007/s11103-017-0629-1

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