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Tyrosine Nitration is a Novel Post-translational Modification Occurring on the Neural Intermediate Filament Protein Peripherin

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Abstract

The biological implication of protein tyrosine nitration in signaling pathways triggered by nitric oxide is recently emerging. Here we report for the first time that nitrotyrosination occurs in the neural intermediate filament protein peripherin. In neuron-like PC12 cells, nitrated peripherin is associated with the cytoskeleton fraction, its level increases during the progression of NGF-induced differentiation and the nitrated protein remains closely associated with stable microtubules. Tyr 17 and Tyr 376 were identified by MALDI-TOF analyses as two specific residues endogenously nitrated. Finally, peripherin nitration is not restricted to PC12 cells but it is also present in vivo in rat brain.

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Acknowledgements

We thank Mr. Francesco Corniola for the assistance in figures preparation. This work was supported by grants from FIRST 2003–2004 (University of Milano).

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Authors and Affiliations

  1. D.I.P.A.V. – Section of Biochemistry, University of Milano, Via Celoria 10, 20100, Milano, Italy

    Gabriella Tedeschi, Simona Nonnis, Francesca Taverna, Armando Negri, Cristina Ronchi & Severino Ronchi

  2. Department of Biology, University of Milano, Via Celoria 26, 20133, Milano, Italy

    Graziella Cappelletti

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  1. Gabriella Tedeschi
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  2. Graziella Cappelletti
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  3. Simona Nonnis
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  4. Francesca Taverna
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  5. Armando Negri
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  6. Cristina Ronchi
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  7. Severino Ronchi
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Correspondence to Gabriella Tedeschi.

Additional information

Gabriella Tedeschi and Graziella Cappelletti contributed equally to this work.

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Tedeschi, G., Cappelletti, G., Nonnis, S. et al. Tyrosine Nitration is a Novel Post-translational Modification Occurring on the Neural Intermediate Filament Protein Peripherin. Neurochem Res 32, 433–441 (2007). https://doi.org/10.1007/s11064-006-9244-2

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  • DOI: https://doi.org/10.1007/s11064-006-9244-2

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