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Cytoskeleton changes following differentiation of N1E-115 neuroblastoma cell line

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Summary.

No systematic approach to detect expression of differentiation-related elements was published so far. The undifferentiated N1E-115 neuroblastoma cell line was switched into a neuronal phenotype by DMSO treatment and used for proteomic experiments. We used two-dimensional gel electrophoresis followed by unambiguous mass spectrometrical identification of proteins to generate a map of cytoskeleton proteins (CPs), i.e., to search for differentiation-related structures. Alpha-actin, actin-like protein 6A, gamma-tubulin complex component 2, tubulin alpha 3/alpha 7, CLIP associating protein 2, B4 integrin interactor homolog were detectable in the undifferentiated cell line exclusively and neuron-specific CPs drebrin and presynaptic density protein 95, actin-related protein 2/3, alpha and beta-centractin, PDZ-domain actin binding protein, actinin alpha 1, profilin II, ezrin, coactosin-like protein, transgelin 2, myosin light polypeptide 6, tubulin alpha 2, 6 and 7, beta tubulin (94% similar with tubulin beta-2), tubulin beta 3, tubulin tyrosine ligase-like protein 1, lamin B1 and keratin 20 were observed in the differentiated cell line only. We herein identified differentiation-related expressional patterns thus providing new evidence for the role of CPs in the process of neuronal differentiation.

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Authors and Affiliations

  1. Department of Pediatrics, Medical University of Vienna, Vienna, Austria

    J.-E. Oh, K. Karlmark Raja, J.-H. Shin, A. Pollak & G. Lubec

  2. Prenatal Diagnosis and Therapy, Department of Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria

    K. Karlmark Raja & M. Hengstschläger

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  1. J.-E. Oh
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  2. K. Karlmark Raja
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  3. J.-H. Shin
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  5. M. Hengstschläger
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Oh, JE., Karlmark Raja, K., Shin, JH. et al. Cytoskeleton changes following differentiation of N1E-115 neuroblastoma cell line. Amino Acids 31, 289–298 (2006). https://doi.org/10.1007/s00726-005-0256-z

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