Skip to main content
SpringerLink
Log in

FFAs and adipokine-mediated regulation of hsa-miR-143 expression in human adipocytes

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Accumulating evidence suggests that microRNAs (miRNAs) play an important role in regulating the pathways in adipose tissue that control processes such as adipogenesis, insulin resistance, and inflammation. MiR-143 is a well-characterized miRNA involved in adipogenesis and may be involved in regulating insulin resistance. Free fatty acids (FFAs) and adipokines, such as tumor necrosis factor-α (TNF-α), leptin, resistin, and interleukin-6 (IL-6), have already been identified as main regulators of obesity and insulin sensitivity. Therefore, we studied the effects of these inflammatory cytokines on the expression of miR-143. FFAs, resistin, and leptin downregulated miR-143 expression in human adipocytes, whereas TNF-α and IL-6 had little effect on miR-143 expression. These results suggest that the expression of miR-143 is affected by a variety of factors that are related to insulin sensitivity. Therefore, miR-143 may be an important mediator in the development of obesity-related insulin resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Jeffery RW, Sherwood NE (2008) Is the obesity epidemic exaggerated? BMJ 7638(336):245

    Article  Google Scholar 

  2. Nagai M, Kuriyama S, Kakizaki M, Ohmori-Matsuda K, Sone T, Hozawa A et al (2012) Impact of obesity, overweight and underweight on life expectancy and lifetime medical expenditures: the Ohsaki Cohort Study. BMJ Open 3(2). doi:10.1136/bmjopen-2012-000940

  3. Boden G (1997) Role of fatty acids in the pathogenesis of insulin resistance and NIDDM. Diabetes 1(46):3–10

    Article  Google Scholar 

  4. Hotamisligil GS, Shargill NS, Spiegelman BM (1993) Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 5091(259):87–91

    Article  Google Scholar 

  5. Cohen B, Novick D, Rubinstein M (1996) Modulation of insulin activities by leptin. Science 5290(274):1185–1188

    Article  Google Scholar 

  6. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM et al (2001) The hormone resistin links obesity to diabetes. Nature 6818(409):307–312

    Article  Google Scholar 

  7. Vozarova B, Weyer C, Hanson K, Tataranni PA, Bogardus C, Pratley RE (2001) Circulating interleukin-6 in relation to adiposity, insulin action, and insulin secretion. Obes Res 7(9):414–417

    Article  Google Scholar 

  8. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2(116):281–297

    Article  Google Scholar 

  9. Bushati N, Cohen SM (2007) microRNA functions. Annu Rev Cell Dev Biol 23:175–205

    Article  PubMed  CAS  Google Scholar 

  10. Alvarez-Garcia I, Miska EA (2005) MicroRNA functions in animal development and human disease. Development 21(132):4653–4662

    Article  Google Scholar 

  11. Ali AS, Ali S, Ahmad A, Bao B, Philip PA, Sarkar FH (2011) Expression of microRNAs: potential molecular link between obesity, diabetes and cancer. Obes Rev 12(12):1050–1062

    Article  PubMed  CAS  Google Scholar 

  12. Kajimoto K, Naraba H, Iwai N (2006) MicroRNA and 3T3-L1 pre-adipocyte differentiation. RNA 9(12):1626–1632

    Article  Google Scholar 

  13. Yi C, Xie WD, Li F, Lv Q, He J, Wu J et al (2011) MiR-143 enhances adipogenic differentiation of 3T3-L1 cells through targeting the coding region of mouse pleiotrophin. FEBS Lett 20(585):3303–3309

    Article  Google Scholar 

  14. Jordan SD, Kruger M, Willmes DM, Redemann N, Wunderlich FT, Bronneke HS et al (2011) Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 4(13):434–446

    Article  Google Scholar 

  15. Zhao YP, Zhang CM, Zhu C, Chen XH, Wang JL, Ji CB et al (2010) NYGGF4 homologous gene expression in 3T3-L1 adipocytes: regulation by FFA and adipokines. Mol Biol Rep 7(37):3291–3296

    Article  Google Scholar 

  16. Neville MJ, Collins JM, Gloyn AL, McCarthy MI, Karpe F (2011) Comprehensive human adipose tissue mRNA and microRNA endogenous control selection for quantitative real-time-PCR normalization. Obesity (Silver Spring) 4(19):888–892

    Article  Google Scholar 

  17. Fernyhough ME, Okine E, Hausman G, Vierck JL, Dodson MV (2007) PPARgamma and GLUT-4 expression as developmental regulators/markers for preadipocyte differentiation into an adipocyte. Domest Anim Endocrinol 4(33):367–378

    Article  Google Scholar 

  18. Spiegelman BM, Flier JS (2001) Obesity and the regulation of energy balance. Cell 4(104):531–543

    Article  Google Scholar 

  19. Goossens GH (2008) The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance. Physiol Behav 2(94):206–218

    Article  Google Scholar 

  20. Qatanani M, Lazar MA (2007) Mechanisms of obesity-associated insulin resistance: many choices on the menu. Genes Dev 12(21):1443–1455

    Article  Google Scholar 

  21. Li H, Zhang Z, Zhou X, Wang Z, Wang G, Han Z (2011) Effects of microRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes. Mol Biol Rep 7(38):4273–4280

    Article  Google Scholar 

  22. Takanabe R, Ono K, Abe Y, Takaya T, Horie T, Wada H et al (2008) Up-regulated expression of microRNA-143 in association with obesity in adipose tissue of mice fed high-fat diet. Biochem Biophys Res Commun 4(376):728–732

    Article  Google Scholar 

  23. Boden G (2011) Obesity, insulin resistance and free fatty acids. Curr Opin Endocrinol Diabetes Obes 2(18):139–143

    Article  Google Scholar 

  24. Maeda K, Uysal KT, Makowski L, Gorgun CZ, Atsumi G, Parker RA et al (2003) Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes 2(52):300–307

    Article  Google Scholar 

  25. Boden G, Chen X (1995) Effects of fat on glucose uptake and utilization in patients with non-insulin-dependent diabetes. J Clin Invest 3(96):1261–1268

    Article  Google Scholar 

  26. Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV et al (2004) MicroRNA-143 regulates adipocyte differentiation. J Biol Chem 50(279):52361–52365

    Article  Google Scholar 

  27. Friedman JM, Halaas JL (1998) Leptin and the regulation of body weight in mammals. Nature 6704(395):763–770

    Article  Google Scholar 

  28. Steppan CM, Lazar MA (2002) Resistin and obesity-associated insulin resistance. Trends Endocrinol Metab 1(13):18–23

    Article  Google Scholar 

  29. Pradhan AD, Manson JE, Rifai N, Buring JE, Ridker PM (2001) C-reactive protein, interleukin 6, and risk of developing type 2 diabetes mellitus. JAMA 3(286):327–334

    Article  Google Scholar 

  30. Sewter CP, Digby JE, Blows F, Prins J, O’Rahilly S (1999) Regulation of tumour necrosis factor-alpha release from human adipose tissue in vitro. J Endocrinol 1(163):33–38

    Article  Google Scholar 

  31. Yamashita A, Soga Y, Iwamoto Y, Yoshizawa S, Iwata H, Kokeguchi S et al (2007) Macrophage-adipocyte interaction: marked interleukin-6 production by lipopolysaccharide. Obesity (Silver Spring) 11(15):2549–2552

    Article  Google Scholar 

Download references

Acknowledgments

This project was supported by grants from the National Key Basic Research Program of China (2013CB530604), the National Natural Science Foundation of China (No. 81100618), the Program for Innovative Research Teams of Jiangsu Province (LJ201108), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Nanjing Technological Development Program (201104013).

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Endocrinology, Second Affiliated Hospital, Nanjing Medical University, 210011, Nanjing, China

    Lingling Zhu & Yibing Lu

  2. State Key Laboratory of Reproductive Medicine, Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No. 123 Tianfei Road, 210029, Nanjing, China

    Chunmei Shi, Chenbo Ji, Guangfeng Xu, Ling Chen, Lei Yang, Ziyi Fu, Xianwei Cui & Xirong Guo

  3. Department of Endocrinology, Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029, Nanjing, China

    Lingling Zhu

Authors
  1. Lingling Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chunmei Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chenbo Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guangfeng Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ling Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ziyi Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xianwei Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yibing Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Xirong Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yibing Lu or Xirong Guo.

Additional information

Lingling Zhu and Chunmei Shi contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, L., Shi, C., Ji, C. et al. FFAs and adipokine-mediated regulation of hsa-miR-143 expression in human adipocytes. Mol Biol Rep 40, 5669–5675 (2013). https://doi.org/10.1007/s11033-013-2668-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-013-2668-2

Keywords

Search

Navigation