Abstract
MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. miRNA-143 has been proposed to play a role in the differentiation of preadipocytes. However, effects and mechanism of miRNA-143 in the differentiation of mammals intramuscular adipocytes is unknown. In this study, the fibroblast-like preadipocytes were cultured from the marbling muscle tissue of holstein steers by the ceiling culture method. The in vitro studies showed that the fibroblast-like preadipocytes could differentiated into mature adipocytes with up-regulated expression of miRNA-143. Furthermore, the transfection of the fibroblast-like preadipocytes with miRNA-143 antisense inhibitor induced a significant suppression of differentiation, and indicated by decreased storage of lipid droplets and down-regulated expression of key adipocytes regulatory genes such as CCAAT/enhancer-binding protein-α (C/EBPα) and fatty acid binding proteins-4 (FABP-4). On the contrary, cells proliferation were increased with miRNA-143 inhibitor transfection. Taken together, our study provide the first evidence for stimulation of endogenous miRNA-143 in the differentiation of bovine intramuscular fat, which in part contribute to the regulated expression of adipocyte genes.
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This work was supported by the National Natural Science Foundation of China (No. 31000994), the Doctoral Program Foundation of Institutions of Higher Education of China (No. 20090097120009) and Nanjing Agricultural University Scientific & Technological Innovation Fund for Youths (No. KJ09012).
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Li, H., Zhang, Z., Zhou, X. et al. Effects of MicroRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes. Mol Biol Rep 38, 4273–4280 (2011). https://doi.org/10.1007/s11033-010-0550-z
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DOI: https://doi.org/10.1007/s11033-010-0550-z