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Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro

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Abstract

To clarify the role of cementoblast in orthodontic-related root resorption, this study was attempted to examine whether murine cementoblast-like cells are responsive to mechanical stress, and how mechanical forces regulate bone sialoprotein (BSP) and osteopontin (OPN) gene expression in these cells in vitro. In this force-loading model, defined and reproducible mechanical loadings of different magnitudes and types were applied up to 24 h. Besides a transitory and reversible change in cell proliferation, remarkable alterations in gene transcription of BSP and OPN were found. BSP mRNA was suppressed by the stresses. Three and six hours-loadings at 2,000 μstrain up-regulated the expression of OPN mRNA, while the other loadings inhibited it. The study also concluded that 4,000 μstrain was likely to exert more influence on cementoblast-like cells than 2,000 μstrain. Furthermore, no obvious evidence indicated the difference between tension and compression. These results suggested that cementoblast-like cells are sensitive to mechanical stress, and may play a role in regulating orthodontic-related root resorption/repair.

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Acknowledgments

National Nature Science Foundation of China funded the study (Grant No. 10572098). Thanks for Prof. Somerman’s generous gift of immortalized murine cementoblast-like cells (OCCM-30). The experiment was performed in State Key Laboratory of Oral Disease (Sichuan University), West China Stomatology Hospital, Sichuan University.

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Authors and Affiliations

  1. State Key Laboratory of Oral Disease, Sichuan University, Chengdu, 610041, People’s Republic of China

    Lan Huang, Yao Meng, Xianglong Han & Ding Bai

  2. Department of Orthodontics, West China Stomatology Hospital, Sichuan University, 14#, 3rd section, Renmin South Road, Chengdu, 610041, People’s Republic of China

    Lan Huang, Yao Meng, Xianglong Han & Ding Bai

  3. Department of Orthodontics, Chongqing Stomatology Hospital, Chongqing Medical University, Chongqing, 400015, People’s Republic of China

    Aishu Ren

  4. Department of Orthodontics, Guangdong Provincial Stomatological Hospital, Guangzhou, 510280, People’s Republic of China

    Lina Bao

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  1. Lan Huang
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Correspondence to Ding Bai.

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Huang, L., Meng, Y., Ren, A. et al. Response of cementoblast-like cells to mechanical tensile or compressive stress at physiological levels in vitro. Mol Biol Rep 36, 1741–1748 (2009). https://doi.org/10.1007/s11033-008-9376-3

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  • DOI: https://doi.org/10.1007/s11033-008-9376-3

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