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A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions

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Abstract

To reveal the linkage relationship between the Ms locus, a restorer-of-fertility gene for cytoplasmic male-sterility (CMS) caused by CMS-S cytoplasm in onion (Allium cepa L.) and previously reported molecular markers linked to the Ms locus, 11 recombinants selected from 4,273 segregating plants originating from the cross between male-sterile maternal and male-fertile paternal lines were analyzed. Results showed that genotypes of a codominant marker, jnurf12, were perfectly matched with the male-fertility phenotypes in all recombinants, but that this marker was not applicable in diverse breeding lines due to multiple band patterns. For the development of more reliable markers, a 12-bp indel was identified from the sequences which were obtained by genome walking, and was used to develop a simple PCR marker which was designated jnurf13. When 104 diverse breeding lines containing CMS-S cytoplasm were analyzed with the jnurf13 marker, male-fertility phenotypes of all breeding lines were perfectly matched with marker genotypes. To our surprise, phenotypes of 153 breeding lines containing CMS-T-like cytoplasm were also matched with genotypes of the jnurf13 marker which was linked to the Ms locus for the CMS-S system. Furthermore, phenotypes of four F2 populations containing CMS-T-like cytoplasm co-segregated perfectly with jnurf13 genotypes. Allelic segregation distortion was detected in two F2 populations using the jnurf13 maker. The results of this study were in conflict with a previous model for inheritance of fertility restoration in the CMS-T system. Therefore, we proposed a new model based on the data analyzed with the jnurf13 marker, which was in linkage disequilibrium with restorer-of-fertility genes for both CMS systems.

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Acknowledgments

This research was supported by the Agriculture Research Center program, Golden Seed Project (Center for Horticultural Seed Development, No 213003-04-1-SB910), and a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ007992). We thank Ji-wha Hur and Jeong-Ahn Yoo for their dedicated technical help.

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  1. Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, Kwangju, 500-757, Korea

    Sunggil Kim

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Correspondence to Sunggil Kim.

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11032_2014_73_MOESM4_ESM.tif

Supplementary Fig. 1. Linkage relationship between the Ms locus and linked molecular markers in diverse breeding lines. Male-fertile and male-sterile phenotypes or genotypes of molecular markers are shown in gray and empty boxes, respectively. (TIFF 130 kb)

11032_2014_73_MOESM5_ESM.tif

Supplementary Fig. 2. Identification of cytoplasm types of four F2 populations using four different molecular markers developed for distinction of onion cytoplasm types. Primer sequences of four molecular markers are shown in Supplementary Table 3. N: normal, T: CMS-T, S: CMS-S. 1:020501, 2:020502, 3:020301, 4:020302. (TIFF 160 kb)

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Kim, S. A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Mol Breeding 34, 769–778 (2014). https://doi.org/10.1007/s11032-014-0073-8

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